REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua Aníbal Cunha, 164, 4099-030 Porto, Portugal.
Meat Sci. 2012 Mar;90(3):836-41. doi: 10.1016/j.meatsci.2011.10.018. Epub 2011 Nov 12.
Meat species identification in food has gained increasing interest in recent years due to public health, economic and legal concerns. Following the consumer trend towards high quality products, game meat has earned much attention. The aim of the present work was to develop a DNA-based technique able to identify hare meat. Mitochondrial cytochrome b gene was used to design species-specific primers for hare detection. The new primers proved to be highly specific to Lepus species, allowing the detection of 0.01% of hare meat in pork meat by polymerase chain reaction (PCR). A real-time PCR assay with the new intercalating EvaGreen dye was further proposed as a specific and fast tool for hare identification with increased sensitivity (1pg) compared to end-point PCR (10pg). It can be concluded that the proposed new primers can be used by both species-specific end-point PCR or real-time PCR to accurately authenticate hare meat.
近年来，由于公众健康、经济和法律等方面的考虑，食品中的肉类物种鉴定越来越受到关注。随着消费者对高质量产品的需求趋势，野味越来越受到关注。本研究旨在开发一种基于 DNA 的技术，能够鉴定野兔的肉。本研究使用线粒体细胞色素 b 基因设计了野兔检测的物种特异性引物。新的引物被证明对兔属具有高度特异性，通过聚合酶链反应（PCR）可以检测到猪肉中 0.01%的野兔肉。进一步提出了一种使用新型嵌入性 EvaGreen 染料的实时 PCR 检测方法，与终点 PCR（10pg）相比，该方法具有更高的灵敏度（1pg），是一种用于鉴定野兔的特异性和快速工具。可以得出结论，所提出的新引物可以通过特定终点 PCR 或实时 PCR 来准确鉴定野兔肉。
