Department of Microbiology, Ohio State University, Columbus, OH 43210, USA.
Bioresour Technol. 2012 Jan;104:424-31. doi: 10.1016/j.biortech.2011.11.013. Epub 2011 Nov 10.
The purpose of this study was to characterize the effect of temperature and cellulosic substrates on fermentative metabolites, H(2) production, and community successions in an anaerobic, cellulolytic consortium, TC60. Pyrosequencing analysis indicated that the consortium was predominated by Thermoanaerobacter and Clostridium spp. Metabolite production was analyzed with four cellulosic substrates at 4 kg/m(3). Triplicate cultures of each substrate were incubated at 50 or 60 °C. The main fermentation products (H(2), CO(2), ethanol, and acetate) were monitored over time. The ANOVA model for production rates showed a significant temperature effect (P<0.05) on all products. Increased temperature promoted higher H(2), CO(2), and ethanol yields while acetate yields were only affected prior to 24h of incubation. In addition to individual effects discerned in the model, ANOVA indicated significant interactions between the substrate and temperature. These interactions have not been previously recognized in the literature for cellulolytic and hydrogen-producing microorganisms.
本研究旨在描述温度和纤维素底物对厌氧纤维素分解菌群落 TC60 中发酵代谢产物、氢气生成和群落演替的影响。焦磷酸测序分析表明，该群落主要由热纤维梭菌属和梭菌属的微生物组成。用四种纤维素底物在 4kg/m3 下进行代谢产物分析。每种底物的重复培养物在 50 或 60°C 下进行孵育。随着时间的推移，监测主要发酵产物（H2、CO2、乙醇和乙酸）的生成。产率的方差分析模型显示，所有产物都受到温度的显著影响（P<0.05）。升高温度可促进更高的 H2、CO2 和乙醇产量，而乙酸产量仅在孵育 24 小时前受到影响。除了模型中辨别的个别影响外，方差分析还表明，底物和温度之间存在显著的相互作用。这些相互作用在以前关于纤维素分解和产氢微生物的文献中尚未得到认可。
