Biological Faculty of M.V. Lomonosov Moscow State University, Moscow, Russia.
PLoS One. 2013 Nov 28;8(11):e83004. doi: 10.1371/journal.pone.0083004. eCollection 2013.
Hydrogen-producing thermophilic cellulolytic microorganisms were isolated from cow faeces. Rates of cellulose hydrolysis and hydrogen formation were 0.2 mM L(-1) h(-1) and 1 mM L(-1) h(-1), respectively. An enzymatic fuel cell (EFC) with a hydrogenase anode was used to oxidise hydrogen produced in a microbial bioreactor. The hydrogenase electrode was exposed for 38 days (912 h) to a thermophilic fermentation medium. The hydrogenase activity remaining after continuous operation under load was 73% of the initial value.
从牛粪中分离出了产氢嗜热纤维素分解微生物。纤维素水解和氢气生成的速率分别为 0.2 mM L(-1) h(-1)和 1 mM L(-1) h(-1)。使用带有氢化酶阳极的酶燃料电池 (EFC) 来氧化微生物生物反应器中产生的氢气。氢化酶电极在热发酵培养基中暴露了 38 天（912 小时）。在连续负载下连续运行后，剩余的氢化酶活性为初始值的 73%。
