程序性细胞死亡蛋白 4（PDCD4）和 miR-21 在尿路上皮癌中的表达。
Expression of programmed cell death protein 4 (PDCD4) and miR-21 in urothelial carcinoma.
机构信息
Department of Urology, University of Bonn, Bonn, Germany.
出版信息
Biochem Biophys Res Commun. 2012 Jan 6;417(1):29-34. doi: 10.1016/j.bbrc.2011.11.035. Epub 2011 Nov 19.
BACKGROUND
We investigated the role of the programmed cell death 4 (PDCD4) tumor suppressor gene in specimens of transitional cell carcinoma and of healthy individuals.
METHODS
PDCD4 immunohistochemical expression was investigated in 294 cases in histologically proven transitional cell carcinoma in different tumorous stages (28 controls, 122 non-muscle invasive urothelial carcinoma, stages Tis-T1, 119 invasive transitional cell carcinoma stages T2-T4 and 25 metastases). MiR-21 expression, an important PDCD4 regulator, was assessed with real-time PCR analysis and showed inverse correlation to tissue PDCD4 expression.
RESULTS
Nuclear and cytoplasmatic PDCD4 immunostaining decreased significantly with histopathological progression of the tumor (p<0001). Controls showed strong nuclear and cytoplasmatic immunohistochemical staining. MiR-21 up regulation in tissue corresponded to PDCD4 suppression.
CONCLUSIONS
These data support a decisive role for PDCD4 down regulation in transitional cell carcinoma and confirm miR-21 as a negative regulator for PDCD4. Additionally, PDCD4 immunohistochemical staining turns out to be a possible diagnostic marker for transitional cell carcinoma.
背景
我们研究了程序性细胞死亡因子 4（PDCD4）肿瘤抑制基因在移行细胞癌标本和健康个体中的作用。
方法
在组织学证实的不同肿瘤阶段（28 例对照，122 例非肌肉浸润性尿路上皮癌，Tis-T1 期，119 例浸润性移行细胞癌 T2-T4 期和 25 例转移）的 294 例移行细胞癌病例中，对 PDCD4 免疫组化表达进行了研究。采用实时 PCR 分析评估了重要的 PDCD4 调节因子 miR-21 的表达，结果显示其与组织 PDCD4 表达呈负相关。
结果
核和胞浆 PDCD4 免疫染色随着肿瘤的组织学进展显著降低（p<0001）。对照组显示强烈的核和胞浆免疫组织化学染色。组织中 miR-21 的上调与 PDCD4 的抑制相对应。
结论
这些数据支持 PDCD4 下调在移行细胞癌中的决定性作用，并证实 miR-21 是 PDCD4 的负调节因子。此外，PDCD4 免疫组织化学染色结果可能成为移行细胞癌的诊断标志物。
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