Ministry of Education Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, Guangxi 541004, China.
Chem Commun (Camb). 2012 Jan 18;48(5):699-701. doi: 10.1039/c1cc16435j. Epub 2011 Dec 1.
We report on a novel strategy to improve microfluidic immunoassay sensitivity by introducing chemiluminescence resonance energy transfer (CRET) into the immunoreactions. The proposed CRET-based immunoassay for estradiol (E2, as a model analyte) is one of the most sensitive immunoassay with a limit of detection at 3.6 × 10(-11) M E2 in a microfluidic format.
我们报告了一种通过将化学发光共振能量转移（CRET）引入免疫反应来提高微流控免疫分析灵敏度的新策略。所提出的基于 CRET 的雌二醇（E2，作为模型分析物）免疫分析是最灵敏的免疫分析之一，在微流控格式下，E2 的检测限达到 3.6×10(-11) M。
