肌球蛋白 VB 失活促进胃癌细胞的侵袭和迁移。
Inactivation of MYO5B promotes invasion and motility in gastric cancer cells.
机构信息
Department of Internal Medicine-Oncology, The First Affiliated Hospital, Zhengzhou University, 1 Western Jianshe Road, Zhengzhou 450052, People's Republic of China.
出版信息
Dig Dis Sci. 2012 May;57(5):1247-52. doi: 10.1007/s10620-011-1989-z. Epub 2011 Dec 2.
BACKGROUND
Loss of cell polarity and tissue disorganisation are hallmarks of cancer. MYO5B mutations disrupt epithelial cell polarity, suggesting that MYO5B may be involved in tumorigenesis.
METHODS
We analyzed MYO5B expression in 70 gastric cancer tissues by immunohistochemistry using a tissue microarray method. Two related proteins, Rab11a and TfR, were also investigated.
RESULTS
We found that the negative rate of MYO5B was 78.6 and 17.1% in gastric cancer and normal gastric tissues (P < 0.001), respectively. The MYO5B expression had a strong relationship with Rab11a expression (P = 0.002). We also found that inactivation by siRNA against MYO5B promoted the proliferation, invasion and migration of gastric cancer cells.
CONCLUSION
The expression of MYO5B was downregulated in gastric cancer and inactivation of MYO5B may contribute to tumorigenesis. Therefore, MYO5B may become an important biomarker for gastric cancer in the future.
背景
细胞极性丧失和组织紊乱是癌症的标志。MYO5B 突变破坏上皮细胞极性，提示 MYO5B 可能参与肿瘤发生。
方法
我们使用组织微阵列方法通过免疫组织化学分析了 70 例胃癌组织中的 MYO5B 表达。还研究了两种相关蛋白 Rab11a 和 TfR。
结果
我们发现 MYO5B 在胃癌和正常胃组织中的阴性率分别为 78.6%和 17.1%（P<0.001）。MYO5B 的表达与 Rab11a 的表达呈强相关性（P=0.002）。我们还发现，针对 MYO5B 的 siRNA 失活可促进胃癌细胞的增殖、侵袭和迁移。
结论
MYO5B 在胃癌中表达下调，MYO5B 的失活可能有助于肿瘤发生。因此，MYO5B 可能成为未来胃癌的一个重要生物标志物。
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