Piljac-Zegarac Jasenka, Samec Dunja, Piljac Ante, Mesić Armin, Tkalcec Zdenko
Department of Molecular Biology, Institute "Ruder Bosković," Bijenicka c. 54, P.O. Box 180, 10000 Zagreb, Croatia.
Int J Med Mushrooms. 2011;13(3):257-63. doi: 10.1615/intjmedmushr.v13.i3.50.
Antioxidant activity and total phenol (TP) content of methanol and water extracts of three wild Croatian mushroom species Auricularia auricula-judae (Bull.) Quél., Sarcoscypha austriaca (Sacc.) Boud., and Strobilurus esculentus (Wulfen) Singer were determined and compared with the values obtained for extracts of four cultivated mushrooms Agaricus bisporus (J.E. Lange) Imbach (brown and white strains), Pleurotus ostreatus (Jacq.) P. Kumm., and Lentinus edodes (Berk.) Singer. Spectrophotometric determination of the TP content was performed using the Folin-Ciocalteu method, while antioxidant activity was measured in a reaction with 1,1-diphenyl-2-picrylhydrazyl radical (DPPH assay) and ferric-tripyridyltriazine (Fe3+-TPTZ) complex (FRAP assay). On the average, 5.8-fold higher TP content was observed for water in comparison to methanol extracts of all analyzed mushrooms. Consequently, antioxidant activity was also higher for water extracts, which is evident from the obtained higher values in the FRAP assay and lower EC50 values in the DPPH assay. Among the three tested wild species, the water extract of S. esculentus exhibited the highest concentration of TP, 8.12 mg/g gallic acid equivalents (GAE), the highest reducing power, 19.42 mmol Fe2+/kg, and the best radical scavenging properties, EC50= 13.5 mg/mL.
测定了克罗地亚三种野生蘑菇木耳（Auricularia auricula-judae (Bull.) Quél.）、奥地利肉杯菌（Sarcoscypha austriaca (Sacc.) Boud.）和美味球盖菇（Strobilurus esculentus (Wulfen) Singer）甲醇提取物和水提取物的抗氧化活性及总酚（TP）含量，并与四种栽培蘑菇双孢蘑菇（Agaricus bisporus (J.E. Lange) Imbach，棕色和白色菌株）、平菇（Pleurotus ostreatus (Jacq.) P. Kumm.）和香菇（Lentinus edodes (Berk.) Singer）提取物的相应值进行比较。采用福林-酚法通过分光光度法测定TP含量，同时通过与1,1-二苯基-2-苦基肼自由基反应（DPPH法）和三吡啶三嗪铁（Fe3+-TPTZ）络合物反应（FRAP法）测定抗氧化活性。平均而言，所有分析蘑菇的水提取物中TP含量比甲醇提取物高5.8倍。因此，水提取物的抗氧化活性也更高，这从FRAP法中获得的较高值和DPPH法中较低的EC50值可以明显看出。在三种测试的野生物种中，美味球盖菇的水提取物表现出最高的TP浓度，8.12 mg/g没食子酸当量（GAE），最高的还原能力，19.42 mmol Fe2+/kg，以及最佳的自由基清除性能，EC50 = 13.5 mg/mL。
