Department of Molecular Biology, Institute Ruder Bosković, Bijenicka c 54, PO Box 180, 10000 Zagreb, Croatia.
Food Chem Toxicol. 2010 Jan;48(1):113-9. doi: 10.1016/j.fct.2009.09.026. Epub 2009 Sep 26.
Antioxidant and antimicrobial activities, as well as total phenol (TP, Folin-Ciocalteu method) and phenolic acid (UPLC-MS/MS) contents of leaf and flower infusions of Teucrium arduini L. from six different mountainous localities in Croatia (Ucka, Vosac, Sveti Jure, Snjeznica, Vaganac, Susanj) were analysed in this study. Antioxidant capacity was evaluated using the ferric reducing/antioxidant power (FRAP) assay, as well as 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2'-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging assays. The antioxidant potency composite index (ACI), giving equal weight to all three methods used to quantify antioxidant capacity, was the highest for the sample from Vosac (96.7) among flower infusions, while maximum ACI (100) was determined for the infusion from Ucka among leaf infusions. Strong positive correlation was found between the total phenols and ACI for leaf (r=0.953) and flower (r=0.977) infusions. Our results point to significantly (p<0.05) different TP content between leaf and flower infusions, as well as across localities. Leaf infusions of T. arduini from Susanj exhibited marked antibacterial activity against Staphylococcus aureus, while none of the tested infusions exhibited antimicrobial activity against gram-negative bacterial species, or the tested fungal species.
本研究分析了来自克罗地亚六个不同山区（乌卡、沃萨茨、圣尤雷、什涅兹尼察、瓦加纳茨和苏桑日）的 Teucrium arduini L. 的叶和花浸剂的抗氧化和抗菌活性，以及总酚（Folin-Ciocalteu 法）和酚酸（UPLC-MS/MS）含量。抗氧化能力通过铁还原/抗氧化能力（FRAP）测定法，以及 2,2-二苯基-1-苦基肼基（DPPH）和 2,2'-联氨-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除测定法进行评估。抗氧化能力综合指数（ACI），对三种用于定量抗氧化能力的方法给予相同的权重，花浸剂中来自 Vosac 的样品最高（96.7），而叶浸剂中来自 Ucka 的样品最高（100）。叶（r=0.953）和花（r=0.977）浸剂中总酚和 ACI 之间存在很强的正相关关系。我们的结果表明，叶和花浸剂以及不同地区之间的总酚含量存在显著差异（p<0.05）。来自苏桑日的 T. arduini 叶浸剂对金黄色葡萄球菌表现出明显的抗菌活性，而测试的浸剂对革兰氏阴性菌或测试的真菌均无抗菌活性。
