University of Michigan-Dearborn Department of Natural Sciences, Dearborn, MI 48128-1491, USA.
Can J Microbiol. 2011 Dec;57(12):1067-72. doi: 10.1139/w11-097. Epub 2011 Dec 2.
Enumerating environmental microbial isolates capable of polycyclic aromatic hydrocarbon (PAH) degradation can provide insight into the microbe-plant interactions that facilitate PAH removal. We examined a known PAH degrader ( Pseudomonas putida G7), a nondegrader ( Agrobacterium tumefaciens LBA4404), and several microorganisms isolated from the environment by using a PAH cocktail in an enumeration medium with or without 0.025% (m/v) glucose and (or) root exudates. Compared with the standard most probable number (MPN), the addition of glucose and root exudates in a modified MPN method resulted in a 3- to 11-fold enhancement of PAH degraders being enumerated among microorganisms found in PAH-contaminated soils. High-performance liquid chromatography analysis verified that PAH levels were reduced using this modified enumeration method. Low levels of glucose, perhaps in concert with other materials in exudates, may promote microbial metabolism, thereby enhancing PAH degradation.
对能够降解多环芳烃（PAH）的环境微生物分离物进行计数，可以深入了解促进 PAH 去除的微生物-植物相互作用。我们使用 PAH 混合物在含有或不含有 0.025%（m/v）葡萄糖和（或）根分泌物的计数培养基中，检查了一种已知的 PAH 降解菌（假单胞菌 G7）、一种非降解菌（根癌农杆菌 LBA4404）和几种从环境中分离的微生物。与标准最大可能数（MPN）相比，在改良的 MPN 方法中添加葡萄糖和根分泌物可使在受 PAH 污染土壤中发现的微生物中计数的 PAH 降解菌增加 3 至 11 倍。高效液相色谱分析证实，使用这种改良的计数方法可以降低 PAH 水平。低水平的葡萄糖，可能与分泌物中的其他物质协同作用，可能会促进微生物代谢，从而增强 PAH 的降解。
