Regional Medical Research Centre (ICMR), Indian Council of Medical Research, Post Bag-13, Port Blair-744 101, Andaman and Nicobar Islands.
Can J Microbiol. 2011 Dec;57(12):1073-7. doi: 10.1139/w11-103. Epub 2011 Dec 2.
Chikungunya virus (CHIKV) is an Alphavirus belonging to the family Togaviridae. In 2006, CHIKV infection struck the Andaman and Nicobar archipelago, with an attack rate of 60%. There were more than 10 cases with acute flaccid paralysis simulating the Guillian Barre Syndrome. The majority of the patients presented severe joint pain. The cause for such an explosive nature of the outbreak with increased morbidity was not known. The isolation of CHIKV was attempted and succeeded from nine subjects presenting clinical symptoms of Chikungunya fever. The cDNA of all the isolates was sequenced for partial E1 and nsP1 genes. Sequences were aligned based on the double locus sequence typing concept. The phylogenetic analysis shows that sequences of Andaman isolates grouped with the East, Central, and South African genotype of virus isolates from India, Sri Lanka, and Réunion. The genetic distance between Andaman isolates and the Réunion isolates was very small. The phylogenetic analysis confirmed the origin of the isolates responsible for the first ever confirmed CHIKV outbreak in these islands to be the East, Central, and South African genotype. In this manuscript, we discuss the involvement of the East, Central, and South African strain with the Chikungunya fever outbreak in this archipelago and double locus sequence typing as a first time approach.
基孔肯雅热病毒（CHIKV）是一种属于披膜病毒科的甲病毒。2006 年，CHIKV 感染袭击了安达曼和尼科巴群岛，发病率为 60%。有 10 多例急性弛缓性麻痹病例模拟吉兰-巴雷综合征。大多数患者出现严重关节疼痛。导致这种爆发性增长且发病率增加的原因尚不清楚。从 9 名出现基孔肯雅热临床症状的患者中尝试并成功分离出 CHIKV。对所有分离株的 cDNA 进行了部分 E1 和 nsP1 基因的测序。根据双基因座序列分型概念对序列进行了比对。系统发育分析表明，安达曼分离株与来自印度、斯里兰卡和留尼汪的东、中、南非基因型的病毒分离株聚在一起。安达曼分离株与留尼汪分离株之间的遗传距离非常小。系统发育分析证实，这些岛屿上首次确诊的 CHIKV 暴发是由东、中、南非基因型引起的。在本文中，我们讨论了东、中、南非株与该群岛基孔肯雅热暴发的关系，以及双基因座序列分型作为首次应用的方法。
