Afreen Nazia, Deeba Farah, Khan Wajihul H, Haider Shakir H, Kazim Syed Naqui, Ishrat Romana, Naqvi Irshad Hussain, Shareef Mohammad Y, Broor Shobha, Ahmed Anwar, Parveen Shama
Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, 110025, India.
Microbiol Immunol. 2014 Dec;58(12):688-96. doi: 10.1111/1348-0421.12209.
Dengue and chikungunya are acute viral infections with overlapping clinical symptoms. Both diseases are transmitted by common mosquito vectors resulting in their co-circulation in a region. Molecular and serological tests specific for both dengue and chikungunya infections were performed on 87 acute phase blood samples collected from patients with suspected dengue/chikungunya infections in Delhi from September to December, 2011. RT-PCR and IgM ELISA were performed to detect dengue virus (DENV) and chikungunya virus (CHIKV). NS1 and IgG ELISA were also performed to detect DENV specific antigen and secondary DENV infection. DENV infection was detected in 49%, CHIKV infection in 29% and co-infection with DENV and CHIKV in 10% of the samples by RT-PCR. DENV serotypes 1, 2 and 3 were detected in this study. Nine DENV-1 strains, six DENV-2 strains and 20 CHIKV strains were characterized by DNA sequencing and phylogenetic analysis of their respective envelope protein genes. DENV-1 strains grouped in the American African genotype, DENV-2 strains in the Cosmopolitan genotype and CHIKV strains in the East Central South African genotype by phylogenetic analysis. This is one of the few studies reporting the phylogeny of two dengue virus serotypes (DENV-1 and DENV-2) and CHIKV. Surveillance and monitoring of DENV and CHIKV strains are important for design of strategies to control impending epidemics.
登革热和基孔肯雅热是具有重叠临床症状的急性病毒感染。这两种疾病均由常见的蚊媒传播，导致它们在一个地区共同传播。对2011年9月至12月从德里疑似登革热/基孔肯雅热感染患者中采集的87份急性期血样进行了针对登革热和基孔肯雅热感染的分子和血清学检测。进行逆转录聚合酶链反应（RT-PCR）和IgM酶联免疫吸附测定（ELISA）以检测登革热病毒（DENV）和基孔肯雅热病毒（CHIKV）。还进行了NS1和IgG ELISA以检测DENV特异性抗原和继发性DENV感染。通过RT-PCR在49%的样本中检测到DENV感染，29%的样本中检测到CHIKV感染，10%的样本中检测到DENV和CHIKV合并感染。在本研究中检测到DENV血清型1、2和3。通过对9株DENV-1、6株DENV-2和20株CHIKV各自包膜蛋白基因进行DNA测序和系统发育分析对其进行了鉴定。通过系统发育分析，DENV-1毒株归为美洲-非洲基因型，DENV-2毒株归为世界基因型，CHIKV毒株归为东中非-南非基因型。这是少数几项报告两种登革热病毒血清型（DENV-1和DENV-2）和CHIKV系统发育的研究之一。对DENV和CHIKV毒株进行监测对于制定控制即将发生的疫情的策略很重要。
