Jia Z, Zhang J, Wu Z, Tian J
Key Laboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Reprod Domest Anim. 2012 Oct;47(5):718-23. doi: 10.1111/j.1439-0531.2011.01949.x. Epub 2011 Dec 3.
We investigated the effects of leptin on the in vitro maturation (IVM) and development of calf oocytes. Cumulus-oocyte complexes were matured in IVM medium containing 0-100 ng/ml leptin. Experiment 1 showed that exposure of calf oocytes to IVM medium containing 1 or 10 ng/ml leptin significantly increased rates of development to the metaphase II stage compared with the control (81.7 ± 3.0% and 83.3 ± 2.1% for 1 and 10 ng/ml leptin, respectively, vs 64.1 ± 5.1% for control; p < 0.05). Experiment 2 showed that 1 or 10 ng/ml leptin significantly improved cleavage rates after in vitro fertilization when compared to control (58.6 ± 3.3% and 59.3 ± 2.9% for 1 and 10 ng/ml leptin, respectively, vs 48.5 ± 2.6% for control; p < 0.05); in addition, when compared to control medium, the addition of 10 ng/ml leptin to the IVM medium resulted in more presumptive zygotes reaching the 4- to 8-cell stage after 48 h of in vitro culture (30.3 ± 2.3% vs 20.1 ± 2.3%; p < 0.05) and developing into blastocysts after 8 days of culture (20.4 ± 1.6% vs 11.7 ± 1.7%; p < 0.05). Experiment 3 showed that the addition of 1 or 10 ng/ml leptin significantly increased the total number of blastocyst cells on day 8 of culture (114.6 ± 7.8 and 117.4 ± 5.9 for 1 and 10 ng/ml leptin, respectively, vs 92.7 ± 8.3 for control; p < 0.05) and trophectoderm (TE) cells (88.5 ± 5.5 and 90.6 ± 3.7 for 1 and 10 ng/ml leptin, respectively, vs 70.1 ± 5.9 for control; p < 0.05). In summary, these results indicate that the addition of leptin to IVM medium enhances meiotic maturation and embryo development from calf oocytes and improves the quality of embryos derived from these oocytes.
我们研究了瘦素对犊牛卵母细胞体外成熟（IVM）及发育的影响。卵丘-卵母细胞复合体在含0 - 100 ng/ml瘦素的IVM培养基中成熟。实验1表明，与对照组相比，将犊牛卵母细胞暴露于含1或10 ng/ml瘦素的IVM培养基中，显著提高了其发育至中期II期的比率（1 ng/ml瘦素组为81.7 ± 3.0%，10 ng/ml瘦素组为83.3 ± 2.1%，对照组为64.1 ± 5.1%；p < 0.05）。实验2表明，与对照组相比，1或10 ng/ml瘦素显著提高了体外受精后的卵裂率（1 ng/ml瘦素组为58.6 ± 3.3%，10 ng/ml瘦素组为59.3 ± 2.9%，对照组为48.5 ± 2.6%；p < 0.05）；此外，与对照培养基相比，在IVM培养基中添加10 ng/ml瘦素，使更多的假定受精卵在体外培养48小时后发育至4 - 8细胞期（30.3 ± 2.3%对20.1 ± 2.3%；p < 0.05），并在培养8天后发育为囊胚（20.4 ± 1.6%对11.7 ± 1.7%；p < 0.05）。实验3表明，添加1或10 ng/ml瘦素显著增加了培养第8天囊胚的细胞总数（1 ng/ml瘦素组为114.6 ± 7.8，10 ng/ml瘦素组为117.4 ± 5.9，对照组为92.7 ± 8.3；p < 0.05）以及滋养外胚层（TE）细胞数量（1 ng/ml瘦素组为88.5 ± 5.5，10 ng/ml瘦素组为90.6 ± 3.7，对照组为70.1 ± 5.9；p < 0.05）。总之，这些结果表明，在IVM培养基中添加瘦素可增强犊牛卵母细胞的减数分裂成熟和胚胎发育，并提高源自这些卵母细胞的胚胎质量。
