Laboratório de Protozoologia, Departamento de Medicina Veterinária Preventiva, Universidade de Londrina - UEL, Postal Box 6001, 86050-970 Londrina, PR, Brazil.
Vet Parasitol. 2012 May 25;186(3-4):216-21. doi: 10.1016/j.vetpar.2011.11.034. Epub 2011 Nov 15.
We evaluated the humoral and cellular immune responses in pigs immunized intranasally with crude rhoptry proteins of Toxoplasma gondii plus Quil-A. The experiment used 13 mixed-breed pigs divided into the following three groups: G1 (vaccinated-challenged, n=6), which received the rhoptry vaccine (200(g/dose); G2 (adjuvant-challenged, n=4), which received PBS plus Quil-A; and G3 (unvaccinated-challenged, n=3), which was the control group. The treatments were performed intranasally at days 0, 21, and 42. Three pigs from G1 produced IgG and IgM antibody levels above the cut-off in the ELISA on the challenge day. Partial protection was observed in G1 at the chronic phase of infection when compared with G3. The preventable fractions were 41.6% and 6.5%, in G1 and G2, respectively. The results of this study suggest that rhoptry proteins plus Quil-A stimulated humoral, local, and systemic immune responses, which were able to partially protect the brain from cyst formation.
我们评估了经鼻给予粗线状体蛋白和 Quil-A 的弓形虫免疫的猪的体液和细胞免疫应答。实验使用了 13 头杂种猪，分为以下三组：G1（接种-攻毒，n=6），给予线状体疫苗（200(g/剂量）；G2（佐剂-攻毒，n=4），给予 PBS 加 Quil-A；G3（未接种-攻毒，n=3），作为对照组。处理在第 0、21 和 42 天经鼻进行。G1 中有 3 头猪在攻毒日的 ELISA 中 IgG 和 IgM 抗体水平超过了临界值。与 G3 相比，G1 在感染的慢性期观察到部分保护。G1 和 G2 的可预防分数分别为 41.6%和 6.5%。本研究结果表明，粗线状体蛋白加 Quil-A 刺激了体液、局部和全身免疫应答，能够部分保护大脑免受囊泡形成的影响。
