Research Group Biology of Testis, Department of Embryology and Genetics, Vrije Universiteit Brussel, Brussels, Belgium.
Fertil Steril. 2012 Feb;97(2):319-23. doi: 10.1016/j.fertnstert.2011.11.009. Epub 2011 Dec 2.
To evaluate the presence of spermatogonia in men diagnosed with Klinefelter syndrome (KS), in whom no testicular spermatozoa were recovered by testicular sperm extraction.
Retrospective case series.
University hospital.
PATIENT(S): Testicular samples from 22 nonmosaic 47,XXY men, aged 24-43 years, with no spermatozoa at multiple biopsies.
INTERVENTION(S): Paraffin-embedded testicular tissue was sectioned and stained with hematoxylin-eosin, and immunostainings were performed for both MAGE-A4 and vimentin.
MAIN OUTCOME MEASURE(S): The presence of spermatogonia.
RESULT(S): Massive fibrosis and hyalinization were observed in all men with KS. Spermatogonia were observed in 4 of 22 men with KS, with differentiation up to the spermatocyte level in 2 of them.
CONCLUSION(S): A few men with KS, having no spermatozoa after testicular sperm extraction, still had few spermatogonia. These patients may eventually benefit from in vitro maturation using spermatogonial stem cells. The adult KS population can thus be divided into three subgroups: one subgroup showing focal spermatogenesis, a second having spermatogonia, and a third group in which no germ cells can be recovered. Further research is necessary to unravel the mechanism leading to these different patterns in patients with KS.
评估诊断为克氏综合征（KS）且在多次睾丸活检中均未发现精子的男性中是否存在精原细胞。
回顾性病例系列。
大学医院。
22 名非嵌合 47,XXY 男性的睾丸样本，年龄 24-43 岁，多次活检均未发现精子。
石蜡包埋的睾丸组织切片，行苏木精-伊红染色，同时进行 MAGE-A4 和波形蛋白免疫染色。
精原细胞的存在。
所有 KS 男性均观察到大量纤维化和玻璃样变。4 名 KS 男性中观察到精原细胞，其中 2 名分化至精母细胞水平。
少数 KS 男性在睾丸精子提取后仍存在少量精原细胞，这些患者最终可能受益于使用精原干细胞的体外成熟。因此，成年 KS 人群可分为三个亚组：一组显示局灶性生精，一组存在精原细胞，第三组无法回收生殖细胞。需要进一步研究以阐明导致 KS 患者出现这些不同模式的机制。
