Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia, PA 19104, United States.
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Feb 1;883-884:136-40. doi: 10.1016/j.jchromb.2011.11.017. Epub 2011 Nov 18.
A method is presented for the measurement of ceramide species in biological fluids using flow injection tandem mass spectrometry. Ceramides are important signaling compounds in a number of cell:cell interactions including apoptosis and neurodegeneration. Because of the large number of potential fatty acid constituent moieties on ceramide molecules, a method which accurately distinguishes different chain-length species was required. The present method does not require HPLC separation and is designed to be applicable to high throughput analysis required for clinical studies. We provide a reference range for all measurable ceramide species in normal human plasma and an example of the utility of the assay in providing biomarkers in an in vitro apoptotic cell death study using murine hematopoietic cells treated with daunorubicin.
本研究提出了一种利用流动注射串联质谱法测量生物体液中神经酰胺种类的方法。神经酰胺是细胞间相互作用（包括细胞凋亡和神经退行性变）中的重要信号化合物。由于神经酰胺分子上可能存在大量的脂肪酸组成部分，因此需要一种能够准确区分不同链长种类的方法。本方法不需要 HPLC 分离，设计用于满足临床研究所需的高通量分析。我们提供了正常人血浆中所有可测量的神经酰胺种类的参考范围，并举例说明了该测定方法在使用柔红霉素处理的鼠造血细胞进行体外细胞凋亡死亡研究中作为生物标志物的应用。
