Department of Chemistry, Wayne State University , 5101 Cass Avenue, Detroit, Michigan 48202, United States.
Gill Center for Biomolecular Science, Indiana University , 702 N. Walnut Grove Avenue, Bloomington, Indiana 47405, United States.
Anal Chem. 2016 Nov 15;88(22):10831-10836. doi: 10.1021/acs.analchem.6b00304. Epub 2016 Nov 3.
Matrix-assisted ionization (MAI)-mass spectrometry (MS) eliminates the need for high voltage, a heat source, lasers, and compressed gases in the ionization process and uses minimal solvents in sample preparation, thus making MAI ideal for field-portable mass spectrometers. The broad applicability of MAI is demonstrated by simple, rapid, and robust positive and negative detection mode analyses of low and high mass compounds including some pesticides, dyes, drugs, lipids, and proteins (186 Da to 8.5 kDa) from various materials including urine, biological tissue sections, paper, and plant material on a low pumping capacity, single-quadrupole mass spectrometer. Different sample introduction methods are applicable, including the use of a pipet tip or glass melting point tube, allowing integration of sample preparation with sample introduction for increased analytical utility and ease of operation, even when sampling directly from surfaces.
基质辅助电离（MAI）-质谱（MS）在电离过程中消除了对高压、热源、激光和压缩气体的需求，并且在样品制备中使用最少的溶剂，因此 MAI 非常适合现场便携式质谱仪。MAI 的广泛适用性通过简单、快速和稳健的正、负检测模式分析得到了证明，包括从小分子到高分子质量化合物的分析，包括一些农药、染料、药物、脂质和蛋白质（186 Da 至 8.5 kDa），这些化合物来自各种材料，包括尿液、生物组织切片、纸张和植物材料，使用低抽气量的单四极杆质谱仪。可以应用不同的样品导入方法，包括使用吸头或玻璃熔点管，允许将样品制备与样品导入集成在一起，以提高分析实用性和操作简便性，即使直接从表面采样也是如此。
