培养的感染海洋拟杆菌的单链 DNA 噬菌体很难用 DNA 结合染色剂检测到。
Cultivated single-stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA-binding stains.
机构信息
Department of Natural Sciences, Linneaus University, Kalmar, Sweden.
出版信息
Appl Environ Microbiol. 2012 Feb;78(3):892-4. doi: 10.1128/AEM.06580-11. Epub 2011 Dec 2.
Abstract
This is the first description of cultivated icosahedral single-stranded DNA (ssDNA) phages isolated on heterotrophic marine bacterioplankton and with Bacteroidetes hosts. None of the 8 phages stained well with DNA-binding stains, suggesting that in situ abundances of ssDNA phages are drastically underestimated using conventional methods for enumeration.
摘要
这是首次描述在异养海洋细菌浮游生物上分离到的具有拟杆菌门宿主的培养二十面体单链 DNA（ssDNA）噬菌体。8 种噬菌体中没有一种能很好地被 DNA 结合染色剂染色，这表明使用传统的计数方法，原位 ssDNA 噬菌体的丰度被大大低估了。
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