Yuan Shengjian, Chen Ling, Liu Quan, Zhou Yan, Yang Jingfang, Deng Deng, Li Hongping, Ma Yingfei
Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518000, China.
Department of Food Science and Engineering, College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen, 518000, China.
Arch Virol. 2018 Jul;163(7):1985-1988. doi: 10.1007/s00705-018-3805-y. Epub 2018 Mar 19.
In this study, two bacteriophage isolates, AhSzq-1 and AhSzw-1 that specifically infect Aeromonas hydrophila strain KT998822, were isolated from seawater and characterized. One-step growth curves showed that the latent period of AhSzq-1 and AhSzw-1 are 50 min and 60 min, respectively. The sequence similarities between AhSzq-1 and AhSzw-1 were 88% at the DNA and 83% at the protein level, suggesting that these two phages are representatives of two different species. The virion morphology, DNA genome size and terminal repeats of these two phages are similar to those of viruses classified as T5virus phages. Both phylogenetic analyses and proteomic comparison show that AhSzq-1 and AhSzw-1 group with members of the T5virus genus. We thus propose these two phages as representative isolates of two new species within the T5virus genus.
在本研究中，从海水中分离出两种特异性感染嗜水气单胞菌KT998822菌株的噬菌体分离株AhSzq-1和AhSzw-1，并对其进行了表征。一步生长曲线表明，AhSzq-1和AhSzw-1的潜伏期分别为50分钟和60分钟。AhSzq-1和AhSzw-1之间的DNA序列相似性为88%，蛋白质水平相似性为83%，表明这两种噬菌体是两个不同物种的代表。这两种噬菌体的病毒体形态、DNA基因组大小和末端重复序列与归类为T5病毒噬菌体的病毒相似。系统发育分析和蛋白质组比较均表明，AhSzq-1和AhSzw-1与T5病毒属的成员归为一类。因此，我们提议将这两种噬菌体作为T5病毒属内两个新物种的代表性分离株。
