绿色荧光蛋白标记的监测工具，用于定量革兰氏阳性菌和革兰氏阴性菌之间的共轭质粒转移。
Green fluorescent protein-labeled monitoring tool to quantify conjugative plasmid transfer between Gram-positive and Gram-negative bacteria.
机构信息
Department of Environmental Microbiology/Genetics, University of Technology, Berlin, Germany.
出版信息
Appl Environ Microbiol. 2012 Feb;78(3):895-9. doi: 10.1128/AEM.05578-11. Epub 2011 Dec 2.
Abstract
On the basis of pIP501, a green fluorescent protein (GFP)-tagged monitoring tool was constructed for quantifying plasmid mobilization among Gram-positive bacteria and between Gram-positive Enterococcus faecalis and Gram-negative Escherichia coli. Furthermore, retromobilization of the GFP-tagged monitoring tool was shown from E. faecalis OG1X into the clinical isolate E. faecalis T9.
摘要
基于 pIP501，构建了一个绿色荧光蛋白（GFP）标记的监测工具，用于定量革兰氏阳性菌之间以及革兰氏阳性粪肠球菌和革兰氏阴性大肠杆菌之间的质粒转移。此外，还显示 GFP 标记监测工具从粪肠球菌 OG1X 反向转移到临床分离株粪肠球菌 T9 中。
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