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粪肠球菌向大肠杆菌的接合性质粒转移。

Conjugative plasmid transfer from Enterococcus faecalis to Escherichia coli.

作者信息

Trieu-Cuot P, Carlier C, Courvalin P

机构信息

Centre National de la Recherche Scientifique Unité Associée 271, Institut Pasteur, Paris, France.

出版信息

J Bacteriol. 1988 Sep;170(9):4388-91. doi: 10.1128/jb.170.9.4388-4391.1988.

DOI:10.1128/jb.170.9.4388-4391.1988
PMID:3137216
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC211457/
Abstract

The possibility of transfer of genetic information by conjugation from gram-positive to gram-negative bacteria was investigated with a pBR322-pAM beta 1 chimeric plasmid, designated pAT191. This shuttle vector, which possesses the tra functions of the streptococcal plasmid pAM beta 1, was conjugatively transferred from Enterococcus faecalis to Escherichia coli with an average frequency of 5 x 10(-9) per donor colony formed after mating.

摘要

利用一种名为pAT191的pBR322 - pAMβ1嵌合质粒,研究了通过接合作用将遗传信息从革兰氏阳性菌转移到革兰氏阴性菌的可能性。这种穿梭载体具有链球菌质粒pAMβ1的转移功能,在交配后形成的每个供体菌落中,它以平均5×10⁻⁹的频率从粪肠球菌接合转移至大肠杆菌。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4eae/211457/846c5d23d1b3/jbacter00187-0610-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4eae/211457/846c5d23d1b3/jbacter00187-0610-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4eae/211457/846c5d23d1b3/jbacter00187-0610-a.jpg

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Improved method for conjugative transfer by filter mating of Streptococcus pneumoniae.通过肺炎链球菌滤膜交配进行接合转移的改进方法。
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Distinct roles of ComK1 and ComK2 in gene regulation in Bacillus cereus.ComK1 和 ComK2 在蜡样芽胞杆菌基因调控中的不同作用。
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Analysis of the mobilization functions of the vancomycin resistance transposon Tn1549, a member of a new family of conjugative elements.分析万古霉素耐药转座子 Tn1549 的动员功能,该转座子属于一个新的可移动共轭元件家族。
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