Veltri R W, McClung J E, Sprinkle P M
IARC Sci Publ (1971). 1978(24 Pt 2):729-32.
A preliminary report on the use of specific rabbit antisera raised to Epstein-Barr virus-coded antigens (EBNA and EA) for detection of these antigens in vivo is presented. Human lymphocytes were isolated on isokinetic gradients and the C3 receptor-bearing B-lymphocyte subpopulation was isolated, providing an enriched source of EBV-infected lymphocytes. Such technology was employed to establish the status of the EBV host-cell complex in recurrent exudative tonsillitis (RET), infectious mononucleosis (IM), and Hodgkin's and non-Hodgkin's lymphoma patients. Only EBNA was detected in the lymphocytes from the tonsils of RET patients and the peripheral blood of IM patients. However, the spleen and lymph-nodes of patients with lymphomas had lymphocytes synthesizing EBNA and EA.
本文呈现了一份关于使用针对爱泼斯坦-巴尔病毒编码抗原（EBNA和EA）产生的特异性兔抗血清在体内检测这些抗原的初步报告。通过等动力学梯度分离人淋巴细胞，并分离出携带C3受体的B淋巴细胞亚群，从而提供了丰富的EB病毒感染淋巴细胞来源。利用该技术确定复发性渗出性扁桃体炎（RET）、传染性单核细胞增多症（IM）以及霍奇金淋巴瘤和非霍奇金淋巴瘤患者中EB病毒宿主细胞复合物的状态。仅在RET患者扁桃体淋巴细胞和IM患者外周血淋巴细胞中检测到EBNA。然而，淋巴瘤患者的脾脏和淋巴结中有合成EBNA和EA的淋巴细胞。
