Coyle Heather Miller
Forensic Science Department, Henry C. Lee College of Criminal Justice and Forensic Science, University of New Haven, West Haven, CT, USA.
Methods Mol Biol. 2012;830:241-51. doi: 10.1007/978-1-61779-461-2_17.
For routine genetic analysis of Cannabis sativa, two methods are currently in use, (a) AFLP; amplified fragment length polymorphism analysis and (b) STR; short tandem repeat analysis. The AFLP method used on capillary electrophoresis instrumentation is fully described in this chapter. AFLP analysis generates numerous nonspecific marker fragments for a complex DNA pattern and is available in kit format for quality assurance of reagents. This method is particularly useful when discerning the genetics of highly inbred plant species that may share much of the same DNA with only slight differences due to their common genetic background. AFLP analysis, however, is most effective on fresh or well-preserved plant specimens where the integrity of the DNA is high and the sample is a single source specimen (i.e., not a mixture of plants or different species).
对于大麻的常规遗传分析，目前使用两种方法：(a)AFLP；扩增片段长度多态性分析，以及(b)STR；短串联重复分析。本章全面介绍了在毛细管电泳仪器上使用的AFLP方法。AFLP分析会产生大量非特异性标记片段，形成复杂的DNA图谱，并且有试剂盒形式可供试剂质量保证使用。当鉴别高度自交植物物种的遗传学特征时，这种方法特别有用，这些植物物种可能由于共同的遗传背景而共享大部分相同的DNA，只是存在细微差异。然而，AFLP分析在新鲜或保存良好的植物标本上最为有效，这些标本DNA完整性高且为单一来源标本（即不是植物混合物或不同物种的混合）。
