Liu Youtan, Tang Jing, Ye Jionxian, Zhan Lifang, Huang Shaonong, Wang Tingting, Gu Miaoning
The University of Hongkong Shenzhen Hospital (Shenzhen Binhai Hospital), Shenzhen 518053, China.
Int J Clin Exp Med. 2011;4(4):276-84. Epub 2011 Oct 19.
This study was designed to investigate the effects of hypertonic/hyperoncotic solution on blood-brain barrier damage, brain edema and morphological changes of rats during whole body hyperthermia. 90 adult male Sprague-Dawley rats were randomized into 5 groups: Control group (a room temperature for 4 hours); Whole body hyperthermia group without solution treatment; Whole body hyperthermia group with Ringer's solution treatment; Whole body hyperthermia group with hydroxyethyl starch and Ringer's solution treatment; Whole body hyperthermia group with Hypertonic/hyperoncotic solution treatment. All rats except those of control group were housed in a heated container and maintained at 36°C for 3 hours until the rectal temperature reached 41-42°C. Corresponding solutions were administered intravenously at the beginning of whole body hyperthermia within 30 minutes as designed. Following whole body hyperthermia, rats were subsequently cooled down for 1h. Evans blue was administered intravenously when the rectal temperature was cooled down to 37°C. The leakage of Evans blue-albumin and water content of brain were calculated and morphological changes were investigated. In group with hypertonic/hyperoncotic solution treatment, brain water content and the leakage of Evans blue-albumin were the lowest among the four whole body hyperthermia groups. Compared with the other three whole body hyperthermia groups, in which profound to moderate damages to blood-brain barrier and brain tissue and cells were found, there were only slight morphological changes in the group with hypertonic/hyperoncotic solutionon treatment. Treatment with hypertonic/hyperoncotic solution appeared to attenuate the injury to blood-brain barrier and reduce brain edema and cell morphological changes in whole body hyperthermia rats.
本研究旨在探讨高渗/高渗胶体溶液对全身热疗期间大鼠血脑屏障损伤、脑水肿及形态学变化的影响。90只成年雄性Sprague-Dawley大鼠随机分为5组：对照组（室温4小时）；未进行溶液处理的全身热疗组；进行林格氏液处理的全身热疗组；进行羟乙基淀粉和林格氏液处理的全身热疗组；进行高渗/高渗胶体溶液处理的全身热疗组。除对照组大鼠外，所有大鼠均置于加热容器中，维持在36°C 3小时，直至直肠温度达到41 - 42°C。按设计在全身热疗开始后30分钟内静脉注射相应溶液。全身热疗后，大鼠随后降温1小时。当直肠温度降至37°C时静脉注射伊文思蓝。计算伊文思蓝-白蛋白渗漏量及脑含水量，并研究形态学变化。在高渗/高渗胶体溶液处理组中，脑含水量及伊文思蓝-白蛋白渗漏量在四个全身热疗组中最低。与其他三个全身热疗组相比，后三组均发现血脑屏障及脑组织和细胞有严重至中度损伤，而高渗/高渗胶体溶液处理组仅有轻微形态学变化。高渗/高渗胶体溶液处理似乎可减轻全身热疗大鼠血脑屏障的损伤，减少脑水肿及细胞形态学变化。
