Cuzon Carlson Verginia C, Mathur Brian N, Davis Margaret I, Lovinger David M
Laboratory for Integrative Neuroscience, National Institute of Alcohol Abuse and Alcoholism, Rockville, MD.
Basal Ganglia. 2011 Nov 1;1(4):201-211. doi: 10.1016/j.baga.2011.11.002.
To characterize GFP-expressing cells in the striatum of Cb6-Tg(Gad1-EGFP)G42Zjh/J mice, in which the Gad1 (also referred to as GAD67) promoter drives GFP expression (Gad1-GFP mouse). BACKGROUND: GFP-expressing cells of the GAD1-GFP mouse have been described to be a population of parvalbumin-positive basket interneurons residing in the cerebral cortex and the cerebellum. However, the cells in the dorsal striatum of these mice have not been characterized. METHODS: Using a combination of immunohistochemistry, electrophysiology, DiI labeling, and retrograde tracing, we investigated the phenotypes of GFP-expressing cells in the GAD1-GFP mice. RESULTS: A small number of striatal neurons express GFP in these mice. In the mature striatum, these cells are preferentially located in the lateral striatum with a strong expression in the lateral striatal streak. The GAD1-GFP positive neurons are distinct from the standard fast-spiking and low-threshold-spiking GAD-67 expressing striatal interneurons and appear to be a subset of medium spiny neurons. These neurons are generally colocalized with striosomal markers such as dynorphin, mu-opioid receptors, as well as CB1 and calretinin-immunopositive fibers. Striatal Gad1-GFP neurons can be separated into two groups based on the shape of the somata and patterns of action potential firing. Retrograde labeling indicated that a proportion of these cells are projection neurons. CONCLUSIONS: The examination of GAD1-GFP cells in these mice revealed 2 subpopulations of ventral striosomal striatal medium spiny neurons, based on morphology, patch-matrix segregation and membrane properties.
鉴定Cb6-Tg(Gad1-EGFP)G42Zjh/J小鼠纹状体中表达绿色荧光蛋白（GFP）的细胞，其中Gad1（也称为GAD67）启动子驱动GFP表达（Gad1-GFP小鼠）。背景：GAD1-GFP小鼠中表达GFP的细胞已被描述为位于大脑皮层和小脑中的一群小白蛋白阳性篮状中间神经元。然而，这些小鼠背侧纹状体中的细胞尚未得到鉴定。方法：我们结合免疫组织化学、电生理学、DiI标记和逆行追踪技术，研究了Gad1-GFP小鼠中表达GFP细胞的表型。结果：在这些小鼠中，少数纹状体神经元表达GFP。在成熟纹状体中，这些细胞优先位于外侧纹状体，在外侧纹状体条纹中表达强烈。GAD1-GFP阳性神经元不同于表达GAD-67的标准快速放电和低阈值放电纹状体中间神经元，似乎是中等棘状神经元的一个亚群。这些神经元通常与纹状体标记物共定位，如强啡肽、μ-阿片受体，以及CB1和钙视网膜蛋白免疫阳性纤维。根据胞体形状和动作电位发放模式，纹状体Gad1-GFP神经元可分为两组。逆行标记表明这些细胞中有一部分是投射神经元。结论：对这些小鼠中GAD1-GFP细胞的研究揭示了腹侧纹状体纹状体中等棘状神经元的两个亚群，基于形态学、斑块-基质分离和膜特性。
