Effect of hydrophilicity of room temperature ionic liquids on the electrochemical and electrocatalytic behaviour of choline oxidase.

In the present report, six different nano-composites contaning the same amine functionalized multi-walled carbon nanotubes (NH(2)-MWCNTs) but different room temperature ionic liquids (RTILs) were prepared. Then, the efficiency of these nano-composites as supporting materials for studying the electrochemistry and electrocatalysis of choline oxidase (ChOx) as a model enzyme were compared. The corresponding cyclic voltammetric and amperometric data showed that the electrocatalytic activity and the electroanalytical performance of immobilized ChOx depends on the degree of hydrophilicity of RTILs used in the applied nano-composite. The higher stability (180 days), higher enzyme loading (6.56 mol cm(-2)), lower detection limit (3.85 μM) and wider linear range (0.005-0.8 mM) was obtained for the most hydrophilic RTIL (1-allyl-3-methylimidazolium bromide).