受X射线照射的小鼠及其后代胸腺淋巴细胞的荧光研究。

Fluorescence study of thymus lymphocytes of X-irradiated mice and their progeny.

作者信息

Sungurov A Y, Ostashevsky I Y

出版信息

Int J Radiat Biol Relat Stud Phys Chem Med. 1979 Jan;35(1):73-82. doi: 10.1080/09553007914550061.

Abstract

The mechanisms of changes in the ultra-violet fluorescence (U.V.F.) intensity of mouse thymus lymphocytes 24 hours and 30 days after whole-body X-irradiation have been studied. The thymus lymphocytes of the first generation offspring (F1) from X-irradiated males and unirradiated females were also investigated. At 24 hours after irradiation the U.V.F. intensity decreased for small doses (50 and 65 rad) and increased for doses of more than 100 rad. The changes in U.V.F. intensity were related to a size-independent mechanism. It was found that the U.V.F. increase for doses of 100-700 rad was not connected with the appearance of non-viable (eosin test) cells. The changes in U.V.F. intensity and cellular composition of the thymus were the same 30 days after irradiation and for F1 mice. The increase in U.V.F. intensity was about 14% and did not depend on dose between 50 and 500 rad. About one-half of this increase was connected with an increase in the proportion of medium and large lymphocytes in the thymus. The rest of the effect was related to a size-independent mechanism.

摘要

对小鼠胸腺淋巴细胞在全身X射线照射后24小时和30天紫外线荧光（U.V.F.）强度变化的机制进行了研究。还对受X射线照射的雄性和未受照射的雌性第一代子代（F1）的胸腺淋巴细胞进行了研究。照射后24小时，小剂量（50和65拉德）时U.V.F.强度降低，而剂量超过100拉德时则升高。U.V.F.强度的变化与一种不依赖细胞大小的机制有关。发现100 - 700拉德剂量时U.V.F.的增加与非存活（伊红试验）细胞的出现无关。照射后30天以及F1小鼠的胸腺中，U.V.F.强度和细胞组成的变化是相同的。U.V.F.强度的增加约为14%，且在50至500拉德之间不依赖于剂量。这种增加的约一半与胸腺中中等和大淋巴细胞比例的增加有关。其余效应与一种不依赖细胞大小的机制有关。