Gapeyev Andrew B, Aripovsky Alexander V, Kulagina Tatiana P
Institute of Cell Biophysics, Russian Academy of Sciences , Pushchino, Moscow Region , Russia.
Int J Radiat Biol. 2015 Mar;91(3):277-85. doi: 10.3109/09553002.2014.980467. Epub 2015 Jan 30.
The effects of extremely high-frequency electromagnetic radiation (EHF EMR) on thymus weight and its fatty acids (FA) content and FA composition in X-irradiated mice were studied to test the involvement of FA in possible protective effects of EHF EMR against ionizing radiation.
Mice were exposed to low-intensity pulse-modulated EHF EMR (42.2 GHz, 0.1 mW/cm(2), 20 min exposure, 1 Hz modulation) and/or X-rays at a dose of 4 Gy with different sequences of the treatments. In 4-5 hours, 10, 30, and 40 days after the last exposure, the thymuses were weighed; total FA content and FA composition of the thymuses were determined on days 1, 10, and 30 using a gas chromatography.
It was shown that after X-irradiation of mice the total FA content per mg of thymic tissue was significantly increased in 4-5 h and decreased in 10 and 30 days after the treatment. On days 30 and 40 after X-irradiation, the thymus weight remained significantly reduced. The first and tenth days after X-rays injury independently of the presence and sequence of EHF EMR exposure were characterized by an increased content of polyunsaturated FA (PUFA) and a decreased content of monounsaturated FA (MUFA) with unchanged content of saturated FA (SFA). Exposure of mice to EHF EMR before or after X-irradiation prevented changes in the total FA content in thymic tissue, returned the summary content of PUFA and MUFA to the control level and decreased the summary content of SFA on the 30th day after the treatments, and promoted the restoration of the thymus weight of X-irradiated mice to the 40th day of the observations.
Changes in the content and composition of PUFA in the early period after treatments as well as at the restoration of the thymus weight under the combined action of EHF EMR and X-rays indicate to an active participation of FA in the acceleration of post-radiation recovery of the thymus by EHF EMR exposure.
研究极高频电磁辐射（EHF EMR）对受X射线照射小鼠胸腺重量及其脂肪酸（FA）含量和FA组成的影响，以检验FA是否参与EHF EMR对电离辐射的可能保护作用。
将小鼠暴露于低强度脉冲调制EHF EMR（42.2 GHz，0.1 mW/cm²，照射20分钟，1 Hz调制）和/或4 Gy剂量的X射线，采用不同的处理顺序。在最后一次照射后的4 - 5小时、10天、30天和40天，称量胸腺重量；在第1天、10天和30天使用气相色谱法测定胸腺的总FA含量和FA组成。
结果表明，小鼠经X射线照射后，每毫克胸腺组织的总FA含量在处理后4 - 5小时显著增加，在10天和30天时降低。在X射线照射后的第30天和40天，胸腺重量仍显著降低。X射线损伤后的第1天和第10天，无论是否存在EHF EMR照射及其照射顺序如何，其特征均为多不饱和脂肪酸（PUFA）含量增加，单不饱和脂肪酸（MUFA）含量降低，饱和脂肪酸（SFA）含量不变。在X射线照射前或后使小鼠暴露于EHF EMR可防止胸腺组织中总FA含量的变化，使PUFA和MUFA的总含量在处理后第30天恢复到对照水平，并降低SFA的总含量，且在观察的第40天促进受X射线照射小鼠胸腺重量的恢复。
处理后早期PUFA含量和组成的变化以及在EHF EMR和X射线联合作用下胸腺重量恢复时的变化表明，FA积极参与了通过EHF EMR照射加速胸腺辐射后恢复的过程。
