Laboratory of Plantation Crops, Institute of Tropical Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
J Basic Microbiol. 2012 Oct;52(5):608-12. doi: 10.1002/jobm.201100308. Epub 2011 Dec 5.
Ergosterol is the main component of the fungal membrane and is not found in plants or other microbial cells. Therefore, it can be a useful biomarker for the quantification of fungal biomass. We are now reporting the first isolation and characterisation of ergosterol from the mycelium of G. boninense. The ergosterol structure was detected by Thin Liquid Chromatography (TLC) and Ultra Performance Liquid Chromatography (UPLC) and confirmed with Gas Chromatography coupled with Mass Spectrometry (GCMS) and Nuclear Magnetic Resonance (NMR) analysis.
麦角甾醇是真菌膜的主要成分，在植物或其他微生物细胞中不存在。因此，它可以作为真菌生物量定量的有用生物标志物。我们现在报告了从 G. boninense 的菌丝体中首次分离和鉴定麦角甾醇。通过薄层层析（TLC）和超高效液相色谱（UPLC）检测到麦角甾醇结构，并通过气相色谱-质谱联用（GCMS）和核磁共振（NMR）分析进行了确认。
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