Sugisaki K, Tsuda T
Department of Internal Medicine, Medical College of Oita, Japan.
Nihon Kyobu Shikkan Gakkai Zasshi. 1990 May;28(5):706-13.
Lymph nodes obtained from 19 patients with sarcoidosis were classified into four groups according to their histological maturation stages, which were the early stage, the former mature stage, the late mature stage and the healing stage. Immunohistochemical analysis of CD4 positive cells and CD8 positive cells were performed using monoclonal antibodies to these lymph nodes. Then the number of the positive cells per square millimeters of a granuloma was estimated using a computerized analytic system for light microscopic figures. The number of CD4-positive cells per square millimeter of a granuloma was almost constant from the early stage to the mature stage, but inclined to decrease in the healing stage; on the other hand, the number of CD8-positive cells per square millimeter of granuloma decreased extremely in the mature stage, compared with the early stage and the healing stage. Moreover the CD4/CD8-positive cell ratio in granulomas, calculated from these data, was elevated in the mature stage, compared with the early and the healing stages. From the above results, the CD4/CD8-positive cell ratio in a granuloma was demonstrated to be a valuable index for evaluating the histological activity of the granuloma of sarcoid lymph nodes. Furthermore, the decrease of CD8-positive cells in mature stage granuloma was considered to be related to the maturation of the epithelioid cell granulomas in sarcoidosis.
从19例结节病患者身上获取的淋巴结，根据其组织学成熟阶段分为四组，即早期、前成熟阶段、后成熟阶段和愈合阶段。使用针对这些淋巴结的单克隆抗体对CD4阳性细胞和CD8阳性细胞进行免疫组织化学分析。然后，使用用于光学显微镜图像的计算机分析系统估计每平方毫米肉芽肿中阳性细胞的数量。每平方毫米肉芽肿中CD4阳性细胞的数量从早期到成熟阶段几乎保持恒定，但在愈合阶段倾向于减少；另一方面，与早期和愈合阶段相比，每平方毫米肉芽肿中CD8阳性细胞的数量在成熟阶段极度减少。此外，根据这些数据计算得出的肉芽肿中CD4/CD8阳性细胞比率在成熟阶段高于早期和愈合阶段。从上述结果来看，肉芽肿中CD4/CD8阳性细胞比率被证明是评估结节病淋巴结肉芽肿组织学活性的一个有价值的指标。此外，成熟阶段肉芽肿中CD8阳性细胞的减少被认为与结节病中上皮样细胞肉芽肿的成熟有关。
