Network analysis and protein function prediction with the PRODISTIN Web site.

Interactions between macromolecules are deciphered to gain information about biological processes and protein function. This information is hidden in large interaction networks, yet very complicated to dissect. In this context, the PRODISTIN Web site is dedicated to the clustering of network proteins according to the identity of their interaction partners, and to the subsequent functional annotation of these clusters. It allows analysing functionally networks and eventually leads to the prediction of function for uncharacterized protein based on their belonging to protein clusters. PRODISTIN analyses also provide an overview of the different biological processes existing in a given interactome. Here, we present a step-by-step procedure to analyse interaction networks using the PRODISTIN Web site. The protocol is illustrated by an application to the Campylobacter jejuni interactome.