Baudot Anaïs, Souiai Ouissem, Brun Christine
Spanish National Cancer Research Centre, Madrid, Spain.
Methods Mol Biol. 2012;804:313-26. doi: 10.1007/978-1-61779-361-5_17.
Interactions between macromolecules are deciphered to gain information about biological processes and protein function. This information is hidden in large interaction networks, yet very complicated to dissect. In this context, the PRODISTIN Web site is dedicated to the clustering of network proteins according to the identity of their interaction partners, and to the subsequent functional annotation of these clusters. It allows analysing functionally networks and eventually leads to the prediction of function for uncharacterized protein based on their belonging to protein clusters. PRODISTIN analyses also provide an overview of the different biological processes existing in a given interactome. Here, we present a step-by-step procedure to analyse interaction networks using the PRODISTIN Web site. The protocol is illustrated by an application to the Campylobacter jejuni interactome.
对大分子之间的相互作用进行解析，以获取有关生物过程和蛋白质功能的信息。这些信息隐藏在大型相互作用网络中，但剖析起来非常复杂。在此背景下，PRODISTIN网站致力于根据网络蛋白质相互作用伙伴的身份对其进行聚类，并对这些聚类进行后续的功能注释。它可以分析功能网络，并最终根据未表征蛋白质所属的蛋白质聚类来预测其功能。PRODISTIN分析还提供了给定相互作用组中存在的不同生物过程的概述。在这里，我们介绍了使用PRODISTIN网站分析相互作用网络的逐步程序。该方案通过应用于空肠弯曲菌相互作用组进行了说明。
