Batt Grégory, Besson Bruno, Ciron Pierre-Emmanuel, de Jong Hidde, Dumas Estelle, Geiselmann Johannes, Monte Regis, Monteiro Pedro T, Page Michel, Rechenmann François, Ropers Delphine
INRIA Paris - Rocquencourt, Domaine de Voluceau, Le Chesnay, France.
Methods Mol Biol. 2012;804:439-62. doi: 10.1007/978-1-61779-361-5_22.
Genetic Network Analyzer (GNA) is a tool for the qualitative modeling and simulation of gene regulatory networks, based on so-called piecewise-linear differential equation models. We describe the use of this tool in the context of the modeling of bacterial regulatory networks, notably the network of global regulators controlling the adaptation of Escherichia coli to carbon starvation conditions. We show how the modeler, by means of GNA, can define a regulatory network, build a model of the network, determine the steady states of the system, perform a qualitative simulation of the network dynamics, and analyze the simulation results using model-checking tools. The example illustrates the interest of qualitative approaches for the analysis of the dynamics of bacterial regulatory networks.
遗传网络分析仪（GNA）是一种基于所谓的分段线性微分方程模型对基因调控网络进行定性建模和模拟的工具。我们描述了该工具在细菌调控网络建模中的应用，特别是在控制大肠杆菌适应碳饥饿条件的全局调控因子网络方面的应用。我们展示了建模者如何通过GNA定义调控网络、构建网络模型、确定系统的稳态、对网络动态进行定性模拟以及使用模型检查工具分析模拟结果。该示例说明了定性方法在分析细菌调控网络动态方面的意义。
