利用秀丽隐杆线虫分析Rho GTP酶在轴突导向中的功能。

Analysis of Rho GTPase function in axon pathfinding using Caenorhabditis elegans.

作者信息

Alan Jamie K, Lundquist Erik A

机构信息

Department of Molecular Biosciences, University of Kansas, Lawrence, KS, USA.

出版信息

Methods Mol Biol. 2012;827:339-58. doi: 10.1007/978-1-61779-442-1_22.

DOI:10.1007/978-1-61779-442-1_22

PMID:22144285

Abstract

We provide information and protocols for the analysis of Rho GTPase function in axon pathfinding in Caenorhabditis elegans. The powerful molecular, genetic, imaging, and transgenic tools available in C. elegans make it an excellent system in which to study the in vivo roles of Rho GTPases. Methods for imaging of axon morphology in Rho GTPase single and double mutants are provided, as well as methods for the construction of transgenic C. elegans strains carrying exogenously introduced transgenes that drive the expression of constitutively active and dominant negative mutants.

摘要

我们提供了用于分析秀丽隐杆线虫轴突导向中Rho GTPase功能的信息和实验方案。秀丽隐杆线虫中可用的强大分子、遗传、成像和转基因工具使其成为研究Rho GTPase体内作用的优秀系统。文中提供了对Rho GTPase单突变体和双突变体轴突形态进行成像的方法，以及构建携带外源导入转基因的秀丽隐杆线虫转基因品系的方法，这些转基因可驱动组成型活性突变体和显性负性突变体的表达。