Xiong Huajiang, Rivero Francisco
Department of Zoophysiology, Zoological Institute, University of Kiel, Kiel, Germany.
Methods Mol Biol. 2012;827:381-92. doi: 10.1007/978-1-61779-442-1_25.
The model organism D. discoideum is well suited to investigate basic questions of molecular and cell biology, particularly those related to the structure, regulation, and dynamics of the cytoskeleton, signal transduction, cell-cell adhesion, and development. D. discoideum makes use of Rho-regulated signaling pathways to reorganize its cytoskeleton during chemotaxis, endocytosis, and cytokinesis. In this organism the Rho family encompasses 20 members, several belonging to the Rac subfamily, but there are no representatives of the Cdc42 and Rho subfamilies. Here we present protocols suitable for monitoring the actin polymerization response and the activation of Rac upon stimulation of aggregation competent cells with the chemoattractant cAMP.
模式生物盘基网柄菌非常适合研究分子和细胞生物学的基本问题，特别是那些与细胞骨架的结构、调节和动力学、信号转导、细胞间粘附以及发育相关的问题。盘基网柄菌在趋化作用、内吞作用和胞质分裂过程中利用Rho调节的信号通路来重组其细胞骨架。在这种生物中，Rho家族包含20个成员，其中几个属于Rac亚家族，但没有Cdc42和Rho亚家族的代表。在这里，我们展示了适用于监测在用趋化因子cAMP刺激聚集感受态细胞时肌动蛋白聚合反应和Rac激活的实验方案。
