Welser Jennifer V, Milner Richard
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, USA.
Methods Mol Biol. 2012;814:81-91. doi: 10.1007/978-1-61779-452-0_6.
Astrocytes play fundamental roles in the establishment and maintenance of tissue homeostasis in the central nervous system (CNS). To examine these different functions in vitro, it is important to be able to generate pure astrocyte cultures. While many "enriched" astrocyte cultures have been described, these are complicated by the presence of other contaminating cell types, such as microglia. In this chapter, we describe a method in which microglia-free astrocyte cultures are generated from neurospheres and also include an immunocytochemical approach to demonstrate the purity of these cultures.
星形胶质细胞在中枢神经系统（CNS）组织稳态的建立和维持中发挥着重要作用。为了在体外研究这些不同功能，能够生成纯星形胶质细胞培养物很重要。虽然已经描述了许多“富集”的星形胶质细胞培养物，但这些培养物因存在其他污染细胞类型（如小胶质细胞）而变得复杂。在本章中，我们描述了一种从神经球生成无微胶质细胞的星形胶质细胞培养物的方法，并且还包括一种免疫细胞化学方法来证明这些培养物的纯度。
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