Gradišnik Lidija, Bošnjak Roman, Bunc Gorazd, Ravnik Janez, Maver Tina, Velnar Tomaž
Faculty of Medicine, Institute of Biomedical Sciences, University of Maribor, Taborska 8, 2000 Maribor, Slovenia.
Alma Mater Europaea ECM, Slovenska 17, 2000 Maribor, Slovenia.
Materials (Basel). 2021 Nov 13;14(22):6857. doi: 10.3390/ma14226857.
In recent decades, cell biology has made rapid progress. Cell isolation and cultivation techniques, supported by modern laboratory procedures and experimental capabilities, provide a wide range of opportunities for in vitro research to study physiological and pathophysiological processes in health and disease. They can also be used very efficiently for the analysis of biomaterials. Before a new biomaterial is ready for implantation into tissues and widespread use in clinical practice, it must be extensively tested. Experimental cell models, which are a suitable testing ground and the first line of empirical exploration of new biomaterials, must contain suitable cells that form the basis of biomaterial testing. To isolate a stable and suitable cell culture, many steps are required. The first and one of the most important steps is the collection of donor tissue, usually during a surgical procedure. Thus, the collection is the foundation for the success of cell isolation. This article explains the sources and neurosurgical procedures for obtaining brain tissue samples for cell isolation techniques, which are essential for biomaterial testing procedures.
近几十年来，细胞生物学取得了飞速进展。在现代实验室操作和实验能力的支持下，细胞分离和培养技术为体外研究提供了广泛的机会，以研究健康和疾病状态下的生理和病理生理过程。它们还能非常有效地用于生物材料分析。在一种新的生物材料准备好植入组织并在临床实践中广泛应用之前，必须进行广泛测试。实验细胞模型是新生物材料合适的测试平台和实证探索的第一线，必须包含构成生物材料测试基础的合适细胞。要分离出稳定且合适的细胞培养物，需要许多步骤。第一步也是最重要的步骤之一是在外科手术过程中采集供体组织。因此，采集是细胞分离成功的基础。本文解释了用于细胞分离技术以获取脑组织样本的来源和神经外科手术程序，这些对于生物材料测试程序至关重要。
