短篇报告：巴西亚马逊地区土著 Tapirapé 族群成员中分离的蓝氏贾第虫的分子特征。
Short report: Molecular characterization of Blastocystis obtained from members of the indigenous Tapirapé ethnic group from the Brazilian Amazon region, Brazil.
机构信息
Department of Biology, Mato Grosso State University, Cáceres, Mato Grosso, Brazil.
出版信息
Am J Trop Med Hyg. 2011 Dec;85(6):1050-3. doi: 10.4269/ajtmh.2011.11-0481.
Abstract
A total of 382 stool samples were examined during a survey of intestinal parasites in members of the Tapirapé ethnic group, who live in the Brazilian Amazon region of Mato Grosso. Fecal DNAs from Blastocystis-positive samples were extracted, polymerase chain reaction amplified using Blastocystis-specific primers targeting the small subunit rRNA gene, and sequenced. Three subtypes (STs) were identified: ST1 (41%), ST2 (32%), and ST3 (17%). Seven mixed infections were found (11%). The subtype distribution was markedly different from that reported in Europe in that ST4 was not detected and ST3 was not the most common subtype. This study is the first to include molecular characterization of Blastocystis in Brazil and in indigenous communities from Latin America.
摘要
在对生活在巴西马托格罗索州亚马逊地区的塔皮拉佩族成员进行肠道寄生虫调查时，共检查了 382 份粪便样本。从粪便阳性样本中提取粪便 DNA，使用针对小亚单位 rRNA 基因的 Blastocystis 特异性引物进行聚合酶链反应扩增，并进行测序。鉴定出三种亚型（ST）：ST1（41%）、ST2（32%）和 ST3（17%）。发现了七种混合感染（11%）。亚型分布与欧洲报道的明显不同，因为未检测到 ST4，并且 ST3 不是最常见的亚型。这项研究首次包括巴西和拉丁美洲土著社区的 Blastocystis 的分子特征。
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