Research Institute for Infectious Diseases of Digestive System, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Department of Medical Parasitology and Mycology & Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran.
Research Institute for Infectious Diseases of Digestive System, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
J Infect Public Health. 2018 Jan-Feb;11(1):43-47. doi: 10.1016/j.jiph.2017.03.009. Epub 2017 Apr 9.
Blastocystis hominis is the most common intestinal parasite found in humans and many other hosts. Pathogenicity of Blastocystis sp. remains controversial and it has been suggested that it may be associated with certain subtypes of organism. The aim of this study was to evaluate the molecular epidemiology of B. hominis and its subtype distribution in Ahvaz, southwest of Iran. During 2012-2014, a total of 481 samples were collected from patients referred to the medical laboratory centers in Ahvaz for stool examination. Samples were examined by wet mount, and genomic DNA was extracted from 50 positive samples. PCR was performed using seven primer pairs targeting the SSU rDNA gene and sequenced. 69 (14.35%) samples were found to be positive for B. hominis and the subtypes of 50 samples were identified. Five subtypes (STs) were identified, including: ST1 (22%), ST2 (6%), ST3 (40%), ST4 (2%), and ST5 (8%). 11 (22%) mixed infections were found, of which 5 were a mixture of ST3/ST4. Mixtures of ST1/ST3 and ST1/ST4 were 3, respectively. In this study people infected with ST3 showed the most gastrointestinal symptoms. This is the first study in the population of Ahvaz and indicates the high prevalence of ST3 in this area. The results suggest a possible association between this subtype and pathogenic potential of parasite.
人芽囊原虫是在人类和许多其他宿主中发现的最常见的肠道寄生虫。芽囊原虫的致病性仍存在争议，有人认为它可能与某些特定的生物体亚型有关。本研究旨在评估伊朗西南部阿瓦士地区人芽囊原虫的分子流行病学及其亚型分布。在 2012 年至 2014 年期间，共从阿瓦士的医疗化验中心就诊的患者中采集了 481 份样本。使用湿片法对样本进行检查，从 50 个阳性样本中提取基因组 DNA。使用针对 SSU rDNA 基因的 7 对引物进行 PCR 扩增，并对产物进行测序。结果发现，69 份（14.35%）样本检测为人芽囊原虫阳性，其中 50 份样本的亚型得到了鉴定。鉴定出 5 种亚型（STs），包括：ST1（22%）、ST2（6%）、ST3（40%）、ST4（2%）和 ST5（8%）。发现 11 例（22%）混合感染，其中 5 例为 ST3/ST4 混合感染。分别有 3 例为 ST1/ST3 和 ST1/ST4 的混合感染。在本研究中，感染 ST3 的人群表现出最多的胃肠道症状。这是在阿瓦士人群中进行的第一项研究，表明该地区 ST3 的高流行率。研究结果提示该亚型可能与寄生虫的致病潜能有关。
