[在西伯利亚和远东地区霍乱流行并发症期间对“杂交”埃尔托型霍乱弧菌菌株的检测]

[Detection of "hybrid" Vibrio cholerae eltor strains during epidemic complications in Syberia and Far East].

作者信息

Mironova L V, Balakhonov S V, Urbanovich L Ia, Polovinkina V S, Kozhevnikova A S, Kulikalova E S, Afanas'ev M V

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 2011 Sep-Oct(5):12-8.

Abstract

AIM

Biotyping of Vibrio cholerae eltor isolated during epidemic complications of cholera in Syberia and Far East by phenotypic and genotypic properties complex.

MATERIALS AND METHODS

45 strains of V. cholerae were studied. Phenotypic analysis was performed by using a complex ofbiovar determining tests. Genotyping was performed by detecting ctxAB, tcpA, toxR, rstRgenes, and ctxB gene structure analysis.

RESULTS

All the V. cholerae during epidemiologic complications in Syberia in the 1970s belong to eltor biovar by phenotypic properties and have eltor specific alleles of tcpA and rstR genes, and ctxB of the third genotype in the genome. In the 1990s the strains were phenotypically matching eltor biovar, but had genetical determinants of both eltor(tcpAE1, rstRE1) and classical (ctxB1, rstR(Cl) biovar.

CONCLUSION

The cause of epidemic complications of cholera in Syberia in the 1970s was V. cholerae eltor with typical eltor biovarphenotypical and genotypical properties. In the 1990s cases of introduction into the region of "hybrid: V. cholerae eltor strain were ascertained, developing into acute cholera outbreaks in several cases.

摘要

目的

通过表型和基因型特性综合分析，对在西伯利亚和远东地区霍乱流行并发症期间分离出的霍乱弧菌埃尔托生物型进行分型。

材料与方法

研究了45株霍乱弧菌。通过一系列生物型鉴定试验进行表型分析。通过检测ctxAB、tcpA、toxR、rstR基因以及对ctxB基因结构进行分析来进行基因分型。

结果

20世纪70年代西伯利亚霍乱流行并发症期间的所有霍乱弧菌，从表型特性来看都属于埃尔托生物型，并且在基因组中具有埃尔托生物型特异性的tcpA和rstR基因等位基因以及第三种基因型(ctxB)。在20世纪90年代，这些菌株在表型上与埃尔托生物型相符，但同时具有埃尔托生物型（tcpAE1、rstRE1）和古典生物型（ctxB1、rstR(Cl)）的基因决定因素。