电迁移分散在毛细管电泳中的作用。
Electromigration dispersion in capillary electrophoresis.
机构信息
Dept. Mech. Eng., Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA.
出版信息
Bull Math Biol. 2012 Feb;74(2):346-55. doi: 10.1007/s11538-011-9708-7. Epub 2011 Dec 7.
Abstract
In a previous paper (Ghosal and Chen in Bull. Math. Biol. 72:2047, 2010), it was shown that the evolution of the solute concentration in capillary electrophoresis is described by a nonlinear wave equation that reduced to Burger's equation if the nonlinearity was weak. It was assumed that only strong electrolytes (fully dissociated) were present. In the present paper, it is shown that the same governing equation also describes the situation where the electrolytic buffer consists of a single weak acid (or base). A simple approximate formula is derived for the dimensionless peak variance which is shown to agree well with published experimental data.
摘要
在之前的一篇论文（Ghosal 和 Chen 在 Bull. Math. Biol. 72:2047, 2010 中）中，已经表明在毛细管电泳中溶质浓度的演化由一个非线性波动方程描述，如果非线性较弱，则该方程简化为 Burger 方程。假设仅存在强电解质（完全离解）。在本文中，证明了相同的控制方程也描述了电解质缓冲液由单一弱酸（或碱）组成的情况。导出了无量纲峰方差的简单近似公式，实验数据表明该公式与已发表的实验数据吻合良好。
相似文献
1
Electromigration dispersion in capillary electrophoresis.电迁移分散在毛细管电泳中的作用。
Bull Math Biol. 2012 Feb;74(2):346-55. doi: 10.1007/s11538-011-9708-7. Epub 2011 Dec 7.
2
Electromigration dispersion in a capillary in the presence of electro-osmotic flow.在存在电渗流的情况下毛细管中的电迁移分散。
J Fluid Mech. 2012 Apr 1;697:436-454. doi: 10.1017/jfm.2012.76. Epub 2012 Mar 9.
3
Strongly nonlinear waves in capillary electrophoresis.毛细管电泳中的强非线性波
Phys Rev E Stat Nonlin Soft Matter Phys. 2012 May;85(5 Pt 1):051918. doi: 10.1103/PhysRevE.85.051918. Epub 2012 May 25.
4
Influence of ignored and well-known zone distortions on the separation performance of proteins in capillary free zone electrophoresis with special reference to analysis in polyacrylamide-coated fused silica capillaries in various buffers. I. Theoretical studies.被忽略和已知的区带畸变对毛细管无区带电泳中蛋白质分离性能的影响，特别涉及在各种缓冲液中聚丙烯酰胺涂层熔融石英毛细管中的分析。I. 理论研究。
J Chromatogr A. 2004 Oct 22;1053(1-2):181-99.
5
Why robust background electrolytes containing multivalent ionic species can fail in capillary zone electrophoresis.为何含有多价离子物种的强背景电解质在毛细管区带电泳中会失效。
J Chromatogr A. 2001 May 4;916(1-2):41-9. doi: 10.1016/s0021-9673(00)01270-x.
6
Conductivity detection in capillary zone electrophoresis: inspection by PeakMaster.毛细管区带电泳中的电导检测：使用PeakMaster进行检测。
Electrophoresis. 2005 May;26(10):1948-53. doi: 10.1002/elps.200410366.
7
Generalized model of the linear theory of electromigration and its application to electrokinetic chromatography: Capillary zone electrophoretic systems with complex-forming equilibria.线性电迁移理论的广义模型及其在电动色谱学中的应用：具有复杂形成平衡的毛细管区带电泳系统。
J Chromatogr A. 2020 Jan 11;1610:460595. doi: 10.1016/j.chroma.2019.460595. Epub 2019 Oct 2.
8
Eigenmobilities in background electrolytes for capillary zone electrophoresis: III. Linear theory of electromigration.毛细管区带电泳背景电解质中的本征淌度：III. 电迁移的线性理论
Electrophoresis. 2004 Oct;25(18-19):3071-9. doi: 10.1002/elps.200405981.
9
Electromigration Dispersion in Sodium Dodecyl Sulfate Capillary Gel Electrophoresis of Proteins.蛋白质在十二烷基硫酸钠胶束毛细管电泳中的电迁移分散。
Anal Chem. 2022 Sep 27;94(38):13092-13099. doi: 10.1021/acs.analchem.2c02348. Epub 2022 Sep 12.
10
Electromigration dispersion in capillary zone electrophoresis. Experimental validation of use of the Haarhoff-Van der Linde function.毛细管区带电泳中的电迁移分散。Haarhoff-Van der Linde函数应用的实验验证。
J Chromatogr A. 2002 Jun 14;959(1-2):229-39. doi: 10.1016/s0021-9673(02)00454-5.
引用本文的文献
1
Peak Integration of Electropherograms in GMP and Research Labs: Navigating Increased Scrutiny Amid Data Integrity Audits and Inspections.GMP和研究实验室中电泳图谱的峰积分：在数据完整性审计和检查日益严格的情况下应对更高的审查要求。
Electrophoresis. 2025 Jun;46(11-12):653-668. doi: 10.1002/elps.70002. Epub 2025 Jun 19.
2
Electrokinetic stacking of particle zones in confined channels enabling their UV absorbance detection on microchips.受限通道中颗粒区带的电动堆积实现其在微芯片上的紫外吸收检测。
Anal Chim Acta. 2020 Oct 23;1135:83-90. doi: 10.1016/j.aca.2020.08.019. Epub 2020 Aug 22.
3
Electromigration dispersion in a capillary in the presence of electro-osmotic flow.在存在电渗流的情况下毛细管中的电迁移分散。
J Fluid Mech. 2012 Apr 1;697:436-454. doi: 10.1017/jfm.2012.76. Epub 2012 Mar 9.
4
Strongly nonlinear waves in capillary electrophoresis.毛细管电泳中的强非线性波
Phys Rev E Stat Nonlin Soft Matter Phys. 2012 May;85(5 Pt 1):051918. doi: 10.1103/PhysRevE.85.051918. Epub 2012 May 25.
本文引用的文献
1
Electromigration dispersion in a capillary in the presence of electro-osmotic flow.在存在电渗流的情况下毛细管中的电迁移分散。
J Fluid Mech. 2012 Apr 1;697:436-454. doi: 10.1017/jfm.2012.76. Epub 2012 Mar 9.
2
Nonlinear waves in capillary electrophoresis.毛细管电泳中的非线性波。
Bull Math Biol. 2010 Nov;72(8):2047-66. doi: 10.1007/s11538-010-9527-2. Epub 2010 Mar 18.
3
Dynamic computer simulations of electrophoresis: three decades of active research.电泳的动态计算机模拟：三十年的活跃研究
Electrophoresis. 2009 Jun;30 Suppl 1:S16-26. doi: 10.1002/elps.200900058.
4
Theory of electrophoresis: fate of one equation.电泳理论：一个方程式的命运
Electrophoresis. 2009 Jun;30 Suppl 1:S7-15. doi: 10.1002/elps.200900133.
5
Fluid mechanics of electroosmotic flow and its effect on band broadening in capillary electrophoresis.毛细管电泳中电渗流的流体力学及其对谱带展宽的影响。
Electrophoresis. 2004 Jan;25(2):214-28. doi: 10.1002/elps.200305745.
6
Free-zone electrophoresis in glass capillaries.玻璃毛细管中的自由区电泳。
Clin Chem. 1981 Sep;27(9):1551-3.
Zotero 插件