Jorgenson J W, Lukacs K D
Clin Chem. 1981 Sep;27(9):1551-3.
A simple theory of zone electrophoresis in open-tubular capillaries is developed. According to this theory, to achieve the highest resolution of zones, tubes with as small an inside diameter as possible should be used in combination with as high an applied voltage as feasible. To test this approach, we performed electrophoresis in glass capillaries with an internal diameter of 75 micron and a length of 100 cm. A special fluorescence detector was used to detect fluorescent zones while they migrated inside the capillary. With the application of 30 kV potentials to this system, rapid and efficient separations of amino acids, peptides, and urinary amines were demonstrated. In all cases fluorescent derivatives were necessary for detection. Preliminary results are encouraging, and with further development of sensitive detection devices, applications in clinical analyses may be feasible.
本文提出了一种关于开管毛细管区带电泳的简单理论。根据该理论，为了实现最高的区带分辨率，应使用内径尽可能小的管子，并结合尽可能高的施加电压。为了验证这种方法，我们在内径为75微米、长度为100厘米的玻璃毛细管中进行了电泳。使用一种特殊的荧光检测器来检测荧光区带在毛细管内迁移时的情况。向该系统施加30 kV的电势后，实现了氨基酸、肽和尿胺的快速高效分离。在所有情况下，检测都需要荧光衍生物。初步结果令人鼓舞，随着灵敏检测设备的进一步发展，在临床分析中的应用可能是可行的。
