Protein Technologies, Inc., Tucson, AZ, USA.
J Pept Sci. 2012 Feb;18(2):88-91. doi: 10.1002/psc.1419. Epub 2011 Dec 7.
The ability to speed up conventional Fmoc solid-phase peptide synthesis (SPPS) has many advantages including increased productivity. One way to speed up conventional Fmoc SPPS is the choice of activator. Recently, several new activators have been introduced into the market, and they were evaluated along with some older activators for their ability to synthesize a range of peptides with shorter and longer reaction times. It was found that HDMC, PyClock, COMU, HCTU, and HATU worked well at shorter reaction times (2 × 1 min), but PyOxim and TFFH only worked well at longer reaction times. The performance of PyBOP at shorter reaction times was poor only for more difficult sequences. These results are important for selecting an appropriate activator for fast SPPS applications.
加快常规 Fmoc 固相肽合成（SPPS）的能力具有许多优势，包括提高生产力。加快常规 Fmoc SPPS 的一种方法是选择激活剂。最近，市场上推出了几种新型激活剂，并对其与一些较旧的激活剂一起合成一系列具有较短和较长反应时间的肽的能力进行了评估。结果发现，HDMC、PyClock、COMU、HCTU 和 HATU 在较短的反应时间（2×1 分钟）下效果很好，但 PyOxim 和 TFFH 仅在较长的反应时间下效果很好。PyBOP 在较短的反应时间下的性能仅对于更困难的序列较差。这些结果对于选择合适的激活剂用于快速 SPPS 应用非常重要。
