Dimock K, Stoltzfus C M
J Biol Chem. 1979 Jul 10;254(13):5591-4.
Cycloleucine (1-aminocyclopentane-1-carboxylic acid) is a potent inhibitor of RNA methylation in B77 sarcoma virus-infected chicken embryo fibroblasts. Under conditions where 40 mM cycloleucine is present, internal N-6-methyladenosine and 5'-terminal cap 2'-O-ribose methylations of poly(A)+ RNA are inhibited greater than 90%. The methylation of the 5'-terminal 7-methylguanosine, however, does not appear to be significantly affected. The poly(A)+ RNA synthesized in cycloleucine-treated cells is transported from the nucleus to the cytoplasm and associates with polyribosomes at rates comparable to poly(A)+ RNA in untreated cells. On the other hand, the transport and utilization of newly synthesized ribosomal RNA in cycloleucine-treated cells is impaired, and the accumulation of mature 18 S and 28 S rRNA is reduced.
环亮氨酸（1-氨基环戊烷-1-羧酸）是B77肉瘤病毒感染的鸡胚成纤维细胞中RNA甲基化的有效抑制剂。在存在40 mM环亮氨酸的条件下，聚腺苷酸（poly(A)+）RNA的内部N-6-甲基腺苷和5'-末端帽2'-O-核糖甲基化受到的抑制大于90%。然而，5'-末端7-甲基鸟苷的甲基化似乎没有受到显著影响。在经环亮氨酸处理的细胞中合成的聚腺苷酸（poly(A)+）RNA从细胞核转运到细胞质，并以与未处理细胞中的聚腺苷酸（poly(A)+）RNA相当的速率与多核糖体结合。另一方面，经环亮氨酸处理的细胞中新合成的核糖体RNA的转运和利用受到损害，成熟的18 S和28 S rRNA的积累减少。
