RNA甲基化与真核生物RNA生物合成的调控：CHO细胞中低甲基化tRNA的加工与利用
RNA methylation and control of eukaryotic RNA biosynthesis: processing and utilization of undermethylated tRNAs in CHO cells.
作者信息
Amalric F, Bachellerie J P, Caboche M
出版信息
Nucleic Acids Res. 1977 Dec;4(12):4357-70. doi: 10.1093/nar/4.12.4357.
Abstract
The role of RNA methylations in the control of tRNA production and utilization for protein biosynthesis has been investigated through a study of the effects in vivo of cycloleucine a specific and potent inhibitor of S adenosyl-methionine mediated methylation. During the cycloleucine treatment, the rate of appearance of newly synthetized tRNAs into the cytoplasm is markedly reduced (about 50%). These molecules are extensively (more than 90%) undermethylated and are integrated into polysomes, but at a slower rate than normally methylated tRNAs.
摘要
通过研究环亮氨酸（一种特异性强效的S-腺苷甲硫氨酸介导甲基化抑制剂）在体内的作用，人们对RNA甲基化在控制用于蛋白质生物合成的tRNA产生和利用中的作用进行了研究。在环亮氨酸处理期间，新合成的tRNA进入细胞质的速率显著降低（约50%）。这些分子甲基化程度极低（超过90%），并整合到多核糖体中，但速率比正常甲基化的tRNA慢。
相似文献
1
RNA methylation and control of eukaryotic RNA biosynthesis: processing and utilization of undermethylated tRNAs in CHO cells.RNA甲基化与真核生物RNA生物合成的调控：CHO细胞中低甲基化tRNA的加工与利用
Nucleic Acids Res. 1977 Dec;4(12):4357-70. doi: 10.1093/nar/4.12.4357.
2
Biosynthesis and utilization of extensively undermethylated poly(A)+ RNA in CHO cells during a cycloleucine treatment.在环亮氨酸处理期间CHO细胞中广泛低甲基化的聚腺苷酸加尾RNA的生物合成与利用
Nucleic Acids Res. 1978 Aug;5(8):2927-43. doi: 10.1093/nar/5.8.2927.
3
RNA methylation and control of eukaryotic RNA biosynthesis. Effects of cycloleucine, a specific inhibitor of methylation, on ribosomal RNA maturation.RNA甲基化与真核生物RNA生物合成的调控。甲基化特异性抑制剂环亮氨酸对核糖体RNA成熟的影响。
Eur J Biochem. 1977 Mar 15;74(1):19-29. doi: 10.1111/j.1432-1033.1977.tb11362.x.
4
Processing and function of undermethylated chicken embryo fibroblast mRNA.低甲基化鸡胚成纤维细胞mRNA的加工与功能
J Biol Chem. 1979 Jul 10;254(13):5591-4.
5
Inhibition of tRNA methylation in vitro and in whole cells by an oncostatic S-adenosyl-homocysteine (SAH) analogue: 5'-deoxy 5'-S-isobutyl adenosine (SIBA).一种抗癌的S-腺苷同型半胱氨酸（SAH）类似物：5'-脱氧5'-S-异丁基腺苷（SIBA）对体外和全细胞中tRNA甲基化的抑制作用。
Nucleic Acids Res. 1978 Aug;5(8):2979-89. doi: 10.1093/nar/5.8.2979.
6
Undermethylated transfer RNA does not support phage RNA-directed in vitro protein synthesis.低甲基化转运RNA不支持噬菌体RNA指导的体外蛋白质合成。
Biochim Biophys Acta. 1976 Jul 2;435(3):251-7. doi: 10.1016/0005-2787(76)90106-4.
7
Diversity in mechanism and function of tRNA methyltransferases.转运RNA甲基转移酶的机制与功能多样性
RNA Biol. 2015;12(4):398-411. doi: 10.1080/15476286.2015.1008358.
8
Theorectical mechanisms for synthesis of carcinogen-induced embryonic proteins: III. The tRNA methylases; methylation mechanism and function.
Med Hypotheses. 1978 Sep-Oct;4(5):497-515. doi: 10.1016/0306-9877(78)90018-x.
9
Activation mode of the eukaryotic m2G10 tRNA methyltransferase Trm11 by its partner protein Trm112.真核生物m2G10 tRNA甲基转移酶Trm11与其伴侣蛋白Trm112的激活模式
Nucleic Acids Res. 2017 Feb 28;45(4):1971-1982. doi: 10.1093/nar/gkw1271.
10
Functional characterization of the human tRNA methyltransferases TRMT10A and TRMT10B.人 tRNA 甲基转移酶 TRMT10A 和 TRMT10B 的功能特征分析。
Nucleic Acids Res. 2020 Jun 19;48(11):6157-6169. doi: 10.1093/nar/gkaa353.
引用本文的文献
1
Expression of targeted ribozyme against telomerase RNA causes altered expression of several other genes in tumor cells.针对端粒酶RNA的靶向核酶的表达导致肿瘤细胞中其他几个基因的表达改变。
Tumour Biol. 2014 Jun;35(6):5539-50. doi: 10.1007/s13277-014-1729-z. Epub 2014 Mar 25.
2
Molecular aspects of the in vivo and in vitro effects of ethionine, an analog of methionine.蛋氨酸类似物乙硫氨酸在体内和体外作用的分子层面研究
Microbiol Rev. 1982 Sep;46(3):281-95. doi: 10.1128/mr.46.3.281-295.1982.
3
Synthesis and behaviour of small RNA species of CHO cells submitted to a heat chick.经受热激的中国仓鼠卵巢细胞中小RNA种类的合成与行为
Nucleic Acids Res. 1981 Apr 10;9(7):1615-25. doi: 10.1093/nar/9.7.1615.
4
Evidence that the methylation inhibitor cycloleucine causes accumulation of a discrete ribosomal RNA precursor in hamster mitochondria.甲基化抑制剂环亮氨酸导致仓鼠线粒体中一种离散核糖体RNA前体积累的证据。
Mol Biol Rep. 1986;11(1):51-5. doi: 10.1007/BF00417596.
5
Biosynthesis and utilization of extensively undermethylated poly(A)+ RNA in CHO cells during a cycloleucine treatment.在环亮氨酸处理期间CHO细胞中广泛低甲基化的聚腺苷酸加尾RNA的生物合成与利用
Nucleic Acids Res. 1978 Aug;5(8):2927-43. doi: 10.1093/nar/5.8.2927.
本文引用的文献
1
The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.用聚丙烯酰胺凝胶电泳法分离高分子量核糖核酸
Biochem J. 1967 Jan;102(1):251-7. doi: 10.1042/bj1020251.
2
Isolation of nucleoli. A method that combines high yield, structural integrity, and biochemical preservation.核仁的分离。一种兼具高产量、结构完整性和生化保存性的方法。
J Cell Biol. 1971 Nov;51(21):563-8. doi: 10.1083/jcb.51.2.563.
3
Isolation of mutants of Escherichia coli lac king 5-methyluracil in transfer ribonucleic acid or 1-methylguanine in ribosomal RNA.分离大肠杆菌转移核糖核酸中缺乏5-甲基尿嘧啶或核糖体核糖核酸中缺乏1-甲基鸟嘌呤的突变体。
J Mol Biol. 1970 Jul 14;51(1):83-100. doi: 10.1016/0022-2836(70)90272-x.
4
5
Isolation and properties of a transfer ribonucleic acid deficient in ribothymidine.一种缺乏核糖胸苷的转移核糖核酸的分离及特性
Biochemistry. 1970 Jul 7;9(14):2823-31. doi: 10.1021/bi00816a011.
6
Expression of the mitochondrial genome in HeLa cells. XIX. Occurrence in mitochondria of polyadenylic acid sequences, "free" and covalently linked to mitochondrial DNA-coded RNA.海拉细胞中线粒体基因组的表达。第十九部分。聚腺苷酸序列在线粒体中的存在情况，“游离”的以及与线粒体DNA编码的RNA共价连接的聚腺苷酸序列。
J Mol Biol. 1974 Jan 15;82(2):151-74. doi: 10.1016/0022-2836(74)90338-6.
7
Methyldeficient mammalian 4s RNA: evidence for L-ethionine-induced inhibition of N6-dimethyladenosine synthesis in rat liver tRNA.甲基缺乏的哺乳动物4s RNA：L-乙硫氨酸诱导大鼠肝脏tRNA中N6-二甲基腺苷合成受抑制的证据。
Nucleic Acids Res. 1974 Feb;1(2):279-88. doi: 10.1093/nar/1.2.279.
8
tRNA synthesis in HeLa cells: a precursor to tRNA and the effects of methionine starvation on tRNA synthesis.HeLa细胞中的tRNA合成：tRNA前体以及甲硫氨酸饥饿对tRNA合成的影响
J Mol Biol. 1969 May 28;42(1):43-56. doi: 10.1016/0022-2836(69)90485-9.
9
Physiological and biochemical studies on the function of 5-methyluridine in the transfer ribonucleic acid of Escherichia coli.关于5-甲基尿苷在大肠杆菌转移核糖核酸中功能的生理生化研究。
J Bacteriol. 1975 Oct;124(1):99-111. doi: 10.1128/jb.124.1.99-111.1975.
10
Methylation and processing of transfer ribonucleic acid in mammalian and bacterial cells.哺乳动物和细菌细胞中转运核糖核酸的甲基化与加工
J Biol Chem. 1975 Mar 25;250(6):2143-9.
Zotero 插件