Hay J B, Abernethy N J, Kalaaji A N, Teare G F, Borron P
Department of Immunology, Sunnybrook Hospital, University of Toronto, Ontario, Canada.
Lymphology. 1990 Jun;23(2):64-72.
It has been known for some time that antigen stimulation can alter lymphocyte traffic patterns and that viruses are particularly potent in this respect; such alterations may be a consequence of host-derived factors. The retention of lymphocytes in lymph nodes can be sustained for several hours with locally administered interferon (IFN)alpha. The extravasation of lymphocytes from blood into non-lymphoid tissues can be induced in the skin with IFN gamma and particularly tumor necrosis factor (TNF)alpha. Recent evidence supports the concept that the migratory capacity of CD4+ cells differs from the capacity of CD8+ cells. Agents (cytokines?) which differentially affect the traffic of these two sub-sets have not yet been described but such a possibility has not been adequately tested. Several new molecules have been defined which alter the interactions between lymphocytes and blood vascular endothelial cells, and these may be important in the critical adhesive event in lymphocyte traffic. In both rat and sheep, it has been possible to cultivate post-capillary endothelial cells from lymphoid tissue, and this may be a helpful approach to studying the mechanisms and molecules involved in adhesion. New cell tracking dyes recently available (Zynaxis Cell Science) permit more significant, long-term studies on the life span of lymphocyte sub-sets and their migratory status. In our experiments, labeled lymphocytes can be followed in vivo for over 30 days. Traffic alterations may explain some of the abnormalities in immunodeficiency states.
一段时间以来，人们已经知道抗原刺激可以改变淋巴细胞的迁移模式，而且病毒在这方面尤其有效；这种改变可能是宿主衍生因子的结果。局部给予干扰素（IFN）α可使淋巴细胞在淋巴结中滞留数小时。在皮肤中，IFNγ尤其是肿瘤坏死因子（TNF）α可诱导淋巴细胞从血液渗入非淋巴组织。最近的证据支持这样一种概念，即CD4 +细胞的迁移能力不同于CD8 +细胞的迁移能力。尚未描述对这两个亚群的迁移有不同影响的因子（细胞因子？），但这种可能性尚未得到充分检验。已经确定了几种新分子，它们可改变淋巴细胞与血管内皮细胞之间的相互作用，而这些分子可能在淋巴细胞迁移的关键黏附事件中起重要作用。在大鼠和绵羊中，都已能够从淋巴组织中培养毛细血管后内皮细胞，这可能是研究黏附相关机制和分子的一种有用方法。最近可用的新型细胞追踪染料（Zynaxis细胞科学公司）使得对淋巴细胞亚群的寿命及其迁移状态进行更有意义的长期研究成为可能。在我们的实验中，标记的淋巴细胞在体内可追踪30多天。迁移改变可能解释免疫缺陷状态中的一些异常情况。
