Department of Chemical Engineering, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan.
ACS Appl Mater Interfaces. 2012 Jan;4(1):411-7. doi: 10.1021/am2014713. Epub 2011 Dec 23.
A copolymer with α-D-mannose (Man) and trimethoxysilane (TMS) units was synthesized for immobilization on siliceous matrices such as a sensor cell and membrane. Immobilization of the trimethoxysilane-containing copolymer on the matrices was readily performed by incubation at high heat. The recognition of lectin by poly(Man-r-TMS) was evaluated by measurement with a quartz crystal microbalance (QCM) and adsorption on an affinity membrane, QCM results showed that the mannose-binding protein, concanavalin A, was specifically bound on a poly(Man-r-TMS)-immobilized cell with a higher binding constant than bovine serum albumin. The amount of concanavalin A adsorbed during permeation through a poly(Man-r-TMS)-immobilized membrane was higher than that through an unmodified membrane. Moreover, the concanavalin A adsorbed onto the poly(Man-r-TMS)-immobilized membrane was recoverable by permeation of a mannose derivative at high concentration.
一种含有α-D-甘露糖（Man）和三甲氧基硅烷（TMS）单元的共聚物被合成出来，用于固定在硅质基质上，如传感器细胞和膜。共聚物中的三甲氧基硅烷单元通过在高温下孵育很容易固定在基质上。通过石英晶体微天平（QCM）测量和亲和膜吸附来评估凝集素对聚（Man-r-TMS）的识别。QCM 结果表明，甘露糖结合蛋白，刀豆球蛋白 A，特异性地结合在聚（Man-r-TMS）固定化的细胞上，其结合常数高于牛血清白蛋白。在通过聚（Man-r-TMS）固定化膜渗透过程中吸附的刀豆球蛋白 A 的量高于通过未修饰膜的吸附量。此外，吸附在聚（Man-r-TMS）固定化膜上的刀豆球蛋白 A 可以通过高浓度甘露糖衍生物的渗透而被回收。
