Graduate School of Engineering, Kyushu University , 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.
ACS Appl Mater Interfaces. 2014 Aug 13;6(15):13234-41. doi: 10.1021/am503003v. Epub 2014 Jul 22.
Biosensors for the detection of proteins and bacteria have been developed using glycopolymer-immobilized metal mesh devices. The trimethoxysilane-containing glycopolymer was immobilized onto a metal mesh device using the silane coupling reaction. The surface shape and transmittance properties of the original metal mesh device were maintained following the immobilization of the glycopolymer. The mannose-binding protein (concanavalin A) could be detected at concentrations in the range of 10(-9) to 10(-6) mol L(-1) using the glycopolymer-immobilized metal mesh device sensor, whereas another protein (bovine serum albumin) was not detected. A detection limit of 1 ng mm(-2) was achieved for the amount of adsorbed concanavalin A. The glycopolymer-immobilized metal mesh device sensor could also detect bacteria as well as protein. The mannose-binding strain of Escherichia coli was specifically detected by the glycopolymer-immobilized metal mesh device sensor. The glycopolymer-immobilized metal mesh device could therefore be used as a label-free biosensor showing high levels of selectivity and sensitivity toward proteins and bacteria.
已开发出使用糖聚合物固定化金属网器件来检测蛋白质和细菌的生物传感器。采用硅烷偶联反应将含三甲氧基硅烷的糖聚合物固定在金属网器件上。固定糖聚合物后，保留了原始金属网器件的表面形状和透光性能。使用糖聚合物固定化金属网器件传感器可以检测到浓度范围在 10(-9) 到 10(-6) mol L(-1) 的甘露糖结合蛋白（刀豆球蛋白 A），而另一种蛋白质（牛血清白蛋白）则无法检测到。对于吸附的刀豆球蛋白 A 的量，检测限达到 1 ng mm(-2)。糖聚合物固定化金属网器件传感器还可以检测细菌和蛋白质。甘露糖结合型大肠杆菌通过糖聚合物固定化金属网器件传感器被特异性检测到。因此，糖聚合物固定化金属网器件可用作对蛋白质和细菌具有高选择性和灵敏度的无标记生物传感器。
