采用化学计量学分析的花色苷测定和代谢组学分析研究蔓越莓（Vaccinium macrocarpon Aiton）品种的植物化学多样性。

Phytochemical diversity of cranberry (Vaccinium macrocarpon Aiton) cultivars by anthocyanin determination and metabolomic profiling with chemometric analysis.

机构信息

University of British Columbia, Okanagan Campus, 3333 University Way, Kelowna, British Columbia V1V 1V7, Canada.

出版信息

J Agric Food Chem. 2012 Jan 11;60(1):261-71. doi: 10.1021/jf2033335. Epub 2011 Dec 23.

DOI:10.1021/jf2033335

Abstract

Originally native to the eastern United States, American cranberry ( Vaccinium macrocarpon Aiton, family Ericaceae) cultivation of native and hybrid varieties has spread across North America. Herein is reported the phytochemical diversity of five cranberry cultivars (Stevens, Ben Lear, Bergman, Pilgrim, and GH1) collected in the Greater Vancouver Regional District, by anthocyanin content and UPLC-TOF-MS metabolomic profiling. The anthocyanin content for biological replicates (n = 5) was determined as 7.98 ± 5.83, Ben Lear; 7.02 ± 1.75, Bergman; 6.05 ± 2.51, GH1; 3.28 ± 1.88, Pilgrim; and 2.81 ± 0.81, Stevens. Using subtractive metabonomic algorithms 6481 compounds were found conserved across all varietals, with 136 (Ben Lear), 84 (Bergman), 91 (GH1), 128 (Pilgrim), and 165 (Stevens) unique compounds observed. Principal component analysis (PCA) did not differentiate varieties, whereas partial least-squares discriminate analysis (PLS-DA) exhibited clustering patterns. Univariate statistical approaches were applied to the data set, establishing significance of values and assessing quality of the models. Metabolomic profiling with chemometric analysis proved to be useful for characterizing metabonomic changes across cranberry varieties.

摘要

原产于美国东部，蔓越莓（Vaccinium macrocarpon Aiton，杜鹃花科）的原生和杂交品种已遍布北美。本文报道了在大温哥华地区收集的五个蔓越莓品种（Stevens、Ben Lear、Bergman、Pilgrim 和 GH1）的植物化学多样性，通过花色苷含量和 UPLC-TOF-MS 代谢组学分析。生物重复（n=5）的花色苷含量分别为 7.98±5.83（Ben Lear）、7.02±1.75（Bergman）、6.05±2.51（GH1）、3.28±1.88（Pilgrim）和 2.81±0.81（Stevens）。使用减法代谢组学算法，在所有品种中发现了 6481 种保守化合物，其中 Ben Lear 有 136 种、Bergman 有 84 种、GH1 有 91 种、Pilgrim 有 128 种、Stevens 有 165 种。主成分分析（PCA）不能区分品种，而偏最小二乘判别分析（PLS-DA）则表现出聚类模式。单变量统计方法应用于数据集，确定了值的显著性并评估了模型的质量。化学计量学分析的代谢组学分析被证明是用于描述蔓越莓品种间代谢组变化的有用方法。