Pastoris O, Dossena M, Allorio F, Vercesi L
Institute of Pharmacology, Science Faculty, University of Pavia, Italy.
Mech Ageing Dev. 1990 Jun;54(3):207-19. doi: 10.1016/0047-6374(90)90051-g.
Metabolite concentrations in gastrocnemius and soleus muscles were compared in young-adult (4 months), mature (12 months) and senescent (24 months) rats after continuous (72 consecutive hours) exposure to normobaric hypoxia or normoxia with the intraperitoneal administration of the vasodilator naftidrofuryl or saline solution for 30 days consecutively before hypoxia. The following metabolites were assessed in gastrocnemius muscle in relation to: (a) energy mediators: ATP, ADP, AMP; (b) energy store: creatine phosphate; (c) anaerobic glycolysis: glycogen, glucose, glucose 6-phosphate, pyruvate, lactate; (d) Krebs' cycle: citrate, alpha-ketoglutarate, malate; (e) free amino acids related to Krebs' cycle: aspartate, glutamate, alanine; and (f) ammonia. In the soleus muscle only ATP, creatine phosphate, glycogen, glucose, glucose 6-phosphate, pyruvate, lactate, citrate, alpha-ketoglutarate malate, aspartate and glutamate were assessed. Aging does not seem to affect soleus and gastrocnemius muscles in the same way. Some gastrocnemius muscle metabolites show linear changes in their concentrations with aging, while for the soleus muscle the only linear change relates to glucose 6-phosphate. As regards the influence of hypoxia on muscular metabolism, all the most important changes observed in metabolite concentrations in comparison with control values take place at the age of 4 and 24 months. Furthermore, as regards naftidrofuryl action, the most important variations observed concern only 4-month-old animals. Finally our data show that only in certain cases has pharmacological treatment been able to modify the influence of hypoxic conditions on the concentration of muscle metabolites, regardless of the age of the animals.
在连续（72小时）暴露于常压缺氧或常氧环境之前，对年轻成年（4个月）、成熟（12个月）和衰老（24个月）大鼠连续30天腹腔注射血管扩张剂萘呋胺酯或生理盐水，之后比较腓肠肌和比目鱼肌中的代谢物浓度。对腓肠肌中的以下代谢物进行了评估：(a) 能量介质：ATP、ADP、AMP；(b) 能量储备：磷酸肌酸；(c) 无氧糖酵解：糖原、葡萄糖、6-磷酸葡萄糖、丙酮酸、乳酸；(d) 三羧酸循环：柠檬酸、α-酮戊二酸、苹果酸；(e) 与三羧酸循环相关的游离氨基酸：天冬氨酸、谷氨酸、丙氨酸；以及(f) 氨。在比目鱼肌中，仅评估了ATP、磷酸肌酸、糖原、葡萄糖、6-磷酸葡萄糖、丙酮酸、乳酸、柠檬酸、α-酮戊二酸、苹果酸、天冬氨酸和谷氨酸。衰老对比目鱼肌和腓肠肌的影响似乎并不相同。腓肠肌中的一些代谢物浓度随衰老呈线性变化，而比目鱼肌中唯一的线性变化与6-磷酸葡萄糖有关。关于缺氧对肌肉代谢的影响，与对照值相比，代谢物浓度中观察到的所有最重要变化都发生在4个月和24个月龄时。此外，关于萘呋胺酯的作用，观察到的最重要变化仅涉及4个月龄的动物。最后，我们的数据表明，仅在某些情况下，药物治疗能够改变缺氧条件对肌肉代谢物浓度的影响，而与动物年龄无关。
