Pastoris O, Foppa P, Catapano M, Dossena M
Institute of Pharmacology, Faculty of Science, University of Pavia, Italy.
Exp Gerontol. 1998 Jun;33(4):303-18. doi: 10.1016/s0531-5565(98)00004-7.
The energy metabolism of the gastrocnemius and soleus muscles in young-adult, mature, and senescent rats was evaluated after 72 h of continuous exposure to normobaric hypoxia or normoxia. The effects of treatment with the alpha-adrenergic antagonist nicergoline were also investigated. In the gastrocnemius muscle we evaluated the concentrations of some significative metabolites involved in anaerobic glycolysis and the Krebs' cycle, free amino acids related to the Krebs' cycle, ammonia, some energy mediators, and the energy store creatine phosphate. In the soleus muscle a selection of these was evaluated. In both muscles aging was similarly characterized by a decrease in muscular creatine phosphate concentration, while the energy mediators and the energy charge potential remained unchanged. Singly, some gastrocnemius muscle metabolites showed linear changes in their concentrations with aging, while for the soleus muscle the only linear change regarded glucose-6-phosphate. Continuous normobaric hypoxia induced greater changes at the age of 4 and 24 months than at 12 months. Chronic treatment with nicergoline modified the influence of hypoxic conditions on muscle metabolites concentrations only in some cases, regardless of the age of the animals. Further investigations are necessary before any firm conclusions can be drawn about the pharmacological activity of nicergoline on hypoxia in aged rats.
在持续暴露于常压缺氧或常氧环境72小时后，对年轻成年、成熟和衰老大鼠的腓肠肌和比目鱼肌的能量代谢进行了评估。还研究了用α-肾上腺素能拮抗剂尼麦角林治疗的效果。在腓肠肌中，我们评估了参与无氧糖酵解和三羧酸循环的一些重要代谢物的浓度、与三羧酸循环相关的游离氨基酸、氨、一些能量介质以及能量储存磷酸肌酸。在比目鱼肌中，对其中一部分进行了评估。在这两种肌肉中，衰老的相似特征是肌肉磷酸肌酸浓度降低，而能量介质和能量电荷电位保持不变。单独来看，腓肠肌的一些代谢物浓度随衰老呈线性变化，而比目鱼肌中唯一的线性变化是6-磷酸葡萄糖。持续常压缺氧在4个月和24个月龄时比12个月龄时引起的变化更大。无论动物年龄如何，尼麦角林的长期治疗仅在某些情况下改变了缺氧条件对肌肉代谢物浓度的影响。在就尼麦角林对老年大鼠缺氧的药理活性得出任何确凿结论之前，还需要进一步研究。
