Fukushima S, Ogawa H, Nishikawa T, Sasagawa S
Department of Hygiene, School of Medicine, Kinki University, Osakasayama, Japan.
Mech Ageing Dev. 1990 Jun;54(3):259-70. doi: 10.1016/0047-6374(90)90055-k.
The unicellular ciliate, paramecium, reproduces by binary fission, but can not continue to divide unlimitedly without sexual reproduction. We examined the clonal life span of Paramecium octaurelia stock 299 cultured in conventional bacterized medium (BM) and a chemically defined medium (DM). The cells that lived in BM divided 300 times. Although the cells in DM divided more slowly, some cells continued to divide more than 100 times. The mean life span of 90 cell lines cultured in BM was 151 +/- 49 fissions and that of 84 cell lines in DM was 68 +/- 28. When some older cells, which had been cultured in DM, were transferred to BM, most of them showed much longer life spans than those remaining in DM. The results showed that the life spans of cell clones were affected by the culture conditions.
单细胞纤毛虫草履虫通过二分裂进行繁殖，但如果没有有性生殖，就不能无限期地持续分裂。我们研究了在传统细菌培养基（BM）和化学成分确定的培养基（DM）中培养的八尾草履虫品系299的克隆寿命。生活在BM中的细胞分裂了300次。虽然DM中的细胞分裂较慢，但一些细胞继续分裂了100多次。在BM中培养的90个细胞系的平均寿命为151±49次分裂，在DM中培养的84个细胞系的平均寿命为68±28次分裂。当一些在DM中培养的较老细胞转移到BM中时，它们中的大多数显示出比留在DM中的细胞长得多的寿命。结果表明，细胞克隆的寿命受培养条件的影响。
